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Isolation and characterization of soil Streptomyces species as potential biological control agents against fungal plant pathogens

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Abstract

The use of antagonist microorganisms against fungal plant pathogens is an attractive and ecologically alternative to the use of chemical pesticides. Streptomyces are beneficial soil bacteria and potential candidates for biocontrol agents. This study reports the isolation, characterization and antagonist activity of soil streptomycetes from the Los Petenes Biosphere Reserve, a Natural protected area in Campeche, Mexico. The results showed morphological, physiological and biochemical characterization of six actinomycetes and their inhibitory activity against Curvularia sp., Aspergillus niger, Helminthosporium sp. and Fusarium sp. One isolate, identified as Streptomyces sp. CACIS-1.16CA showed the potential to inhibit additional pathogens as Alternaria sp., Phytophthora capsici, Colletotrichum sp. and Rhizoctonia sp. with percentages ranging from 47 to 90 %. This study identified a streptomycete strain with a broad antagonist activity that could be used for biocontrol of plant pathogenic fungi.

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Acknowledgments

I would like to thank Dr. Jairo Cristobal Alejo from Instituto Tecnológico de Conkal and Dr. Alberto Uc Várguez from CIATEJ-Unidad Sureste, Yucatán, Mexico, for providing fungal strains. I am grateful to Dr. Elizabeth Sugg of Peace Corps Mexico and Dr. Luis Servín-González from Instituto de Investigaciones Biomédicas, UNAM for the critical review, comments and English editing on the manuscript. I acknowledge the collaboration of Dra Ingrid M. Rodríguez-Buenfil from CIATEJ-Unidad Sureste and Dr Carlos Montalvo Romero from Universidad Autónoma del Carmen. This study was supported by grant No. CAMP-2008-C01-96874 (FOMIX Conacyt-Campeche: Isolation, Identification and Conservation of Germplasm Bank of Actinomycetes from Los Petenes Biosphere Reserve). I do not have conflicts of interest to declare.

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  1. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Unidad Sureste, Calle 30 No. 151 (7 y 7A). Col. García Ginerés, C.P. 97070, Mérida, Yucatán, México

    Zahaed Evangelista-Martínez

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Evangelista-Martínez, Z. Isolation and characterization of soil Streptomyces species as potential biological control agents against fungal plant pathogens. World J Microbiol Biotechnol 30, 1639–1647 (2014). https://doi.org/10.1007/s11274-013-1568-x

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  • DOI: https://doi.org/10.1007/s11274-013-1568-x

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