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Molecular characterization of a short peptidoglycan recognition protein (PGRP-S) from Asian corn borer (Ostrinia furnacalis) and its role in triggering proPO activity

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Abstract

Peptidoglycan recognition proteins (PGRPs) are non-specific immune molecules of insects, and vertebrates etc., but are not present in plants and nematodes. In the current experiment, a PGRP DNA sequence (2,910 bp containing four exons) was identified from genomic DNA library of Asian corn borer, Ostrinia furnacalis, and a full-length cDNA programming PGRP was cloned (designed as OfPGRP-S) with an open reading frame of 579 bp, having 192 amino acid. This inferred amino acid sequence showed maximum similarity to known lepidopteran PGRPs. Quantitative real-time PCR investigation disclosed the level of mRNA of OfPGRP-S to be constitutively expressed in the whole developmental stages and with higher expression in the mature larvae. Even more the OfPGRP-S was mainly expressed in immune capable organs i.e., fat body and midgut, and was strongly induced by injecting gram-positive bacteria i.e., Staphylococus aureus. Recombinant protein OfPGRP-S could bind to S. aureus and Bacillus thuringiensis which enhance proPO activation in the presence of these microbes. The results indicated that OfPGRP-S is an inducible protein acting as a receptor-type PGRP for enhancing the proPO activation on exposure to bacteria.

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References

  • Bischoff V, Boneca VC, Michel IG, Hoffmann T, Royet JA (2004) Function of the drosophila pattern-recognition receptor PGRP-SD in the detection of Gram-positive bacteria. Nat Immunol 5:1175–1180

    Article  CAS  Google Scholar 

  • Dziarski R, Gupta D (2006) The peptidoglycan recognition proteins (PGRPs). Genome Biol 7:232

    Article  Google Scholar 

  • Garver LS, Wu J, Wu LP (2006) The peptidoglycan recognition protein PGRP-SC1a is essential for toll signaling and phagocytosis of Staphylococcus aureus in Drosophila. Proc Natl Acad Sci USA 103:660–665

    Article  CAS  Google Scholar 

  • Gelius E, Persson C, Karlsson J, Steiner H (2003) A mammalian peptidoglycan recognition protein with N-acetylmuramoy l-alanine amidase activity. Biochem Biophy Res Commun 306:988–994

    Article  CAS  Google Scholar 

  • Gillespie JP, Kanost MR, Trenczek T (1997) Biological mediators of insect immunity. Annu Rev Entomol 42:611–643

    Article  CAS  Google Scholar 

  • Gupta D, Kirkland TN, Viriyakosol S, Dziarski R (1996) CD14 is a cell-activating receptor for bacterial peptidoglycan. J Biol Chem 271:23310–23316

    Article  CAS  Google Scholar 

  • Hoffmann JA (2003) The immune response of Drosophila. Nature 426:33–38

    Article  CAS  Google Scholar 

  • Hultmark D (1993) Immune reactions in Drosophila and other insects: a model for innate immunity. Trends Genet 9:178–183

    Article  CAS  Google Scholar 

  • Inohara N, Ogura Y, Nunez G (2002) Nods: a family of cytosolic proteins that regulate the host response to pathogens. Curr Opin Microbiol 5:76–80

    Article  CAS  Google Scholar 

  • Kaneko T, Yano T, Aggarwal K, Lim JH, Ueda K, Oshima Y, Peach C, Erturk-Hasdemir D, Goldman WE, Oh BH (2006) PGRP-LC and PGRP-LE have essential yet distinct functions in the drosophila immune response to monomeric DAP-type peptidoglycan. Nat Immunol 7:715–723

    Article  CAS  Google Scholar 

  • Kang D, Liu G, Lundstrom A, Gelius E, Steiner H (1998) A peptidoglycan recognition protein in innate immunity conserved from insects to humans. Proc Natl Acad Sci USA 95:10078–10082

    Article  CAS  Google Scholar 

  • Lee MH, Osaki T, Lee JY, Baek MJ, Zhang R, Park JW, Kawabata S, Derh LK, Lee BL (2004) Peptidoglycan recognition proteins involved in 1, 3-β-d-glucan-dependent prophenoloxidase activation system of insect. J Biol Chem 279:3218–3227

    Article  CAS  Google Scholar 

  • Lemaitre B, Hoffmann J (2007) The host defense of Drosophila melanogaster. Annu Rev Immunol 25:697–743

    Article  CAS  Google Scholar 

  • Lim JH, Kim MS, Kim HE, Yano T, Oshima Y, Aggarwal K, Goldman WE, Silverman N, Kurata S, Oh BH (2006) Structural basis for preferential recognition of diaminopimelic acid-type peptidoglycan by a subset of peptidoglycan recognition proteins. J Biol Chem 281(12):8286–8295

    Google Scholar 

  • Liu C, Xu Z, Gupta D, Dziarski R (2001) Peptidoglycan recognition proteins: a novel family of four human innate immunity pattern recognition molecules. J Biol Chem 276:34686–34694

    Article  CAS  Google Scholar 

  • Medzhitov R, Janeway CA (2002) Decoding the patterns of self and non self by the innate immune system. Science 296:298–300

    Article  CAS  Google Scholar 

  • Mellroth P, Steiner H (2006) PGRP-SB1: an N-acetylmuramoyl l-alanine amidase with antibacterial activity. Biochem Biophys Res Commun 350:994–999

    Article  CAS  Google Scholar 

  • Mellroth P, Karlsson J, Steiner H (2003) A scavenger function for a Drosophila peptidoglycan recognition protein. J Biol Chem 278:7059–7064

    Article  CAS  Google Scholar 

  • Michel T, Reichhart JM, Hoffmann JA, Royet J (2001) Drosophila toll is activated by gram-positive bacteria through a circulating peptidoglycan recognition protein. Nat Rev Immunol 2:756–759

    Google Scholar 

  • Ono H, Matsumoto A, Hashimoto K, Yamano Y, Morishima I (2007) Peptidoglycan recognition protein (PGRP) from eri-silkworm, Samia cynthia ricini; protein purification and induction of the gene expression. Comp Biochem Physiol B Biochem Mol Biol 147:512–519

    Article  Google Scholar 

  • Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477

    CAS  Google Scholar 

  • Sumathipala N, Jiang H (2010) Involvement of Manduca sexta peptidoglycan recognition protein-1 in the recognition of bacteria and activation of prophenoloxidase system. Insect Biochem Mol Biol 40:487–495

    Article  CAS  Google Scholar 

  • Takehana A, Katsuyama T, Yano T, Oshima Y, Takada H, Aigaki T, Kurata S (2002) Overexpression of a pattern-recognition receptor, peptidoglycan-recognition protein-LE, activates imd/relish-mediated antibacterial defense and the prophenoloxidase cascade in Drosophila larvae. Proc Nat Acad Sci USA 99:13705–13710

    Article  CAS  Google Scholar 

  • Takeuch O, Akira S (2001) Toll-like receptors; their physiological role and signal transduction system. Int Immunopharm 1:625–635

    Article  Google Scholar 

  • Wang ZM, Li X, Cocklin RR, Wang M, Fukase K, Inamura S, Kusumoto S, Gupta D, Dziarski R (2003) Human peptidoglycan recognition protein-L is an N-acetylmuramoyl-l-alanine amidase. J Biol Chem 278:49044–49052

    Article  CAS  Google Scholar 

  • Werner T, Liu G, Kang D, Ekengren S, Steiner H, Hultmark D (2000) A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster. Proc Nat Acad Sci USA 97:13772–13777

    Article  CAS  Google Scholar 

  • Werner T, Borge-Renberg K, Mellroth P, Steiner H, Hultmark D (2003) Functional diversity of the Drosophila PGRP-LC gene cluster in the response to lipopolysaccharide and peptidoglycan. J Biol Chem 278:26319–26322

    Article  CAS  Google Scholar 

  • Yoshida H, Kinoshita K, Ashida M (1996) Purification of a peptidoglycan recognition protein from hemolymph of the silkworm, Bombyx mori. J Biol Chem 271:13854–13860

    Article  CAS  Google Scholar 

  • You H, Wan H, Jianhong L, Byung RJ (2010) Molecular cloning and characterization of a short peptidoglycan recognition protein (PGRP-S) with antibacterial activity from the bumblebee Bombus ignites. Dev Comp Immunol 34:977–985

    Article  CAS  Google Scholar 

  • Yu XQ, Zhu YF, Ma C, Fabrick JA, Kanost MR (2002) Pattern recognition proteins in Manduca sexta plasma. Insect Biochem Mol Biol 32:1287–1293

    Article  CAS  Google Scholar 

  • Zhou DR, Wang YY, Liu BL, Ju ZL (1980) Research on the reproduction of Ostrina furnacalis in a large quantity: an artificial diet and its improvement. J Plant Prot 7:113–122

    Google Scholar 

Download references

Acknowledgments

This work was supported by grant from the National Natural Science Foundation of China (31071734; 30900943) and the National Postdoctoral Foundation of China (20080430838).

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Authors and Affiliations

  1. Engineering Research Center of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, 510642, China

    Qiang Sun, Xiao-Xia Xu, Wan-Jun Huang, Zhihua Zheng, Shuang Wang, Shun-Xiang Ren & Feng-Liang Jin

  2. Department of Entomology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, 60800, Pakistan

    Shoaib Freed

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  1. Qiang Sun
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  2. Xiao-Xia Xu
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  3. Shoaib Freed
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  4. Wan-Jun Huang
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  5. Zhihua Zheng
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  6. Shuang Wang
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  7. Shun-Xiang Ren
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Correspondence to Feng-Liang Jin.

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Qiang Sun and Xiao-Xia Xu have contributed equally to this work.

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Sun, Q., Xu, XX., Freed, S. et al. Molecular characterization of a short peptidoglycan recognition protein (PGRP-S) from Asian corn borer (Ostrinia furnacalis) and its role in triggering proPO activity. World J Microbiol Biotechnol 30, 263–270 (2014). https://doi.org/10.1007/s11274-013-1449-3

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  • DOI: https://doi.org/10.1007/s11274-013-1449-3

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