World Journal of Microbiology and Biotechnology

, Volume 29, Issue 12, pp 2437–2440 | Cite as

Identification of a potential fungal species by 18S rDNA for ligninases production

  • M. Ferhan
  • S. N. Santos
  • I. S. Melo
  • N. Yan
  • M. Sain
Short Communication

Abstract

Fungal species for ligninases production was investigated by 18S ribosomal DNA sequence analysis. Two primer sets were chosen to amplify a major part of the 18S rDNA, which resulted in intense PCR product of approximately 550–820 bp in size per sample. The results suggest that the 18S rDNA-based approach is a useful tool for identification of unknown potential fungal species for ligninases production. The isolated fungal species produces mainly manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related polyphenols. Time course studies showed that maximum production of ligninolytic enzymes MnP (64 IU L−1), lignin peroxidase (26.35 IU L−1), and laccase (5.44 IU L−1), respectively, were achieved after 10 days of cultivation under optimum conditions. Furthermore, the biological decolorization of Remazol Brilliant Blue R dye following 10 days of cultivation was 94 %. NCBI BLAST was used to search for closest matched sequences in the GenBank database and based on sequence homology the first BLAST hit was Dothioraceae sp. LM572 with accession number EF060858.1.

Keywords

Dothideomycetes 18S rDNA PCR Ligninases Ascomycetes 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • M. Ferhan
    • 1
    • 2
  • S. N. Santos
    • 2
  • I. S. Melo
    • 2
  • N. Yan
    • 1
  • M. Sain
    • 1
  1. 1.Faculty of Forestry, Centre for Biocomposites and Biomaterials Processing (CBBP)University of TorontoTorontoCanada
  2. 2.Laboratório de Microbiologia Ambiental—Embrapa Meio Ambiente (CNPMA)JaguariúnaBrazil

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