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Expression of catalase in Lactobacillus fermentum and evaluation of its anti-oxidative properties in a dextran sodium sulfate induced mouse colitis model

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Abstract

Lactic acid bacteria are generally sensitive to hydrogen peroxide (H2O2). Lactobacillus plantarum ATCC14431 is one of the few lactic acid bacteria able to degrade H2O2 through the action of a manganese-dependent catalase (containing the katA gene). However, it is not a natural inhabitant of the intestinal tract and its bio-efficacy and survival in the gastrointestinal tract have never been tested. In this study, we successfully expressed the katA gene from L. plantarum ATCC14431 in L. fermentum I5007 and the recombinant L. fermentum exhibited almost 20-fold higher catalase activity than the empty vector control. The anti-oxidative properties of this catalase-producing L. fermentum were evaluated using a dextran sodium sulphate (DSS) induced colitis mice model. Compared with the control, mice receiving DSS alone had increased diarrhea and mucosa histological scores (P < 0.05), as well as lipid peroxidation (P < 0.05), myeloperoxidase (P < 0.05), and active NF-κB in colonic tissue (P < 0.05). Similar to vitamin E, treatment with recombinant L. fermentum mitigate these effects accompanied by a improvement in mucosa histological scores in the proximal colon (P < 0.05) and decreased lipid peroxidation (P < 0.05), myeloperoxidase (P < 0.05) and active NF-κB in colonic tissue (P < 0.05). In conclusion, the expression of catalase in L. fermentum increased its ability to survive when exposed to aerated environment in vitro and conferred the anti-oxidative and anti-inflammatory effects in the DSS induced colitis model.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China PR (NO. 30930066) and National Key Basic Research Program of China (973 Program) 2012CB124702.

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Authors and Affiliations

  1. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China

    Jiang Zhang, Hong Liu, Qingwei Wang, Chengli Hou & Shiyan Qiao

  2. Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada

    Philip Thacker

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  1. Jiang Zhang
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  2. Hong Liu
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  3. Qingwei Wang
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  4. Chengli Hou
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  5. Philip Thacker
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Correspondence to Shiyan Qiao.

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Supplementary material 2 The capability of three different promoters to drive green fluorescent protein expression (DOCX 191 kb)

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Zhang, J., Liu, H., Wang, Q. et al. Expression of catalase in Lactobacillus fermentum and evaluation of its anti-oxidative properties in a dextran sodium sulfate induced mouse colitis model. World J Microbiol Biotechnol 29, 2293–2301 (2013). https://doi.org/10.1007/s11274-013-1395-0

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  • DOI: https://doi.org/10.1007/s11274-013-1395-0

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