Abstract
A recombinant arginase was generated for a whole-cell biotransformation system to convert l-arginine to l-ornithine in Escherichia coli. The gene ARG1 coding arginase from Bos taurus liver was synthesized and expressed in E. coli BL21 (DE3) via pETDuet-1. The recombinant arginase was used to catalyze l-arginine to l-ornithine and urea. The reaction was optimal at pH 9.5 and 37 °C. Manganese (10−5 M) and Emulsifier OP-10 [0.033 % (v/v)] could promote arginase activity. In a scale up study, l-arginine conversion rate reached 98 % with a final concentration of 111.52 g l-ornithine/l.
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This work was supported by Jiangsu Planned Projects for Postdoctoral Research Funds (1101005C), Special Research of Environmental Nonprofit Industry (201009023) and the Open Fund of State Key Laboratory of Pharmaceutical Biotechnology of Nanjing University, China.
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Zhan, Y., Liu, J., Mao, P. et al. Biotransformation of l-ornithine from l-arginine using whole-cell recombinant arginase. World J Microbiol Biotechnol 29, 2167–2172 (2013). https://doi.org/10.1007/s11274-013-1382-5
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DOI: https://doi.org/10.1007/s11274-013-1382-5