Skip to main content
SpringerLink
Log in

A fusant of Sphingomonas sp. GY2B and Pseudomonas sp. GP3A with high capacity of degrading phenanthrene

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

A fusant strain F14 with high biodegradation capability of phenanthrene was obtained by protoplast fusion between Sphingomonas sp. GY2B (GenBank DQ139343) and Pseudomonas sp. GP3A (GenBank EU233280). F14 was screened and identified from 39 random fusants by antibiotic tests, scanning electron microscope (SEM) and randomly amplified polymorphic DNA (RAPD). The result of SEM analysis demonstrated that the cell shape of fusant F14 different from parental strains. RAPD analysis of 5 primers generated a total of 70 bands. The genetic similarity indices between F14 and parental strains GY2B and GP3A were 27.9 and 34.6 %, respectively. F14 could rapidly degrade phenanthrene within 24 h, and the degradation efficiency was much better than GY2B and GP3A. GC–MS analysis of metabolites of phenanthrene degradation indicated F14 had a different degradation pathway from GY2B. Furthermore, the fusant strain F14 had a wider adaptation of temperatures (25–36 °C) and pH values (6.5–9.0) than GY2B. The present study indicated that fusant strain F14 could be an effective and environment-friendly bacterial strain for PAHs bioremediation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Beltrametti F, Barucco D, Rossi R, Selva E, Marinelli F (2007) Protoplast fusion and gene recombination in the uncommon actinomycete Planobispora rosea producing ge2270. J Antibiot 60:447–454

    Article  CAS  Google Scholar 

  • Carney MW, Erwin K, Hardman R, Yuen B, Volz DC, Hinton DE, Kullman SW (2008) Differential developmental toxicity of naphthoic acid isomers in medaka (Oryzias latipes) embryos. Mar Pollut Bull 57:255–266

    Article  CAS  Google Scholar 

  • Chakraborty U, Sikdar SR (2008) Production and characterization of somatic hybrids raised through protoplast fusion between edible mushroom strains Volvariella volvacea and Pleurotus florida. World J Microbiol Biotechnol 24:1481–1492

    Article  Google Scholar 

  • Chen XP, Yi XY, Tao XQ, Wu RR, Yang C, Dang Z (2008) Screening and characterization of pyrene-degrading microbial. Chin J Environ Eng 3:413–417

    Google Scholar 

  • Ghosal D, Chakraborty J, Khara P, Dutta TK (2010) Degradation of phenanthrene via meta-cleavage of 2-hydroxy-1-naphthoic acid by Ochrobactrum sp. strain PWTJD. FEMS Microbiol Lett 313:103–110

    Article  CAS  Google Scholar 

  • Guo C, Zhou H, Wong Y, Tam N (2005) Isolation of PAH-degrading bacteria from mangrove sediments and their biodegradation potential. Mar Pollut Bull 51:1054–1061

    Article  CAS  Google Scholar 

  • Jacques RJS, Santos EC, Bento FM, Peralba MCR, Selbach PA, Sá ELS, Camargo FAO (2005) Anthracene biodegradation by Pseudomonas sp. isolated from a petrochemical sludge landfarming site. Int Biodeterior Biodegradation 56:143–150

    Article  CAS  Google Scholar 

  • Janbandhu A, Fulekar M (2011) Biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment. J Hazard Mater 187:333–340

    Article  CAS  Google Scholar 

  • John RP, Gangadharan D, Madhavan Nampoothiri K (2008) Genome shuffling of Lactobacillus delbrueckii mutant and Bacillus amyloliquefaciens through protoplasmic fusion for L-lactic acid production from starchy wastes. Bioresour Technol 99:8008–8015

    Article  CAS  Google Scholar 

  • Kawa-Rygielska J (2004) Obtaining hybrids of distillery yeasts characterized by the ability of ferementing starch. Biotechnology 7:2

    Google Scholar 

  • Kim TJ, Lee EY, Kim YJ, Cho KS, Ryu HW (2003) Degradation of polyaromatic hydrocarbons by Burkholderia cepacia 2A–12. World J Microbiol Biotechnol 24(19):411–417

    Article  Google Scholar 

  • Kiyohara H, Nagao K, Yana K (1982) Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates. Appl Environ Microbiol 43:454–457

    CAS  Google Scholar 

  • Lu J, Dang Z, Lu GN, Yang C, Yi XY, Guo CL (2012) Biodegradation kinetics of phenanthrene by a fusant strain. Curr Microbiol 65:225–230

    Article  CAS  Google Scholar 

  • Mallick S, Dutta TK (2008) Kinetics of phenanthrene degradation by Staphylococcus sp. Strain PN/Y involving 2-hydroxy-1-naphthoic acid in a novel metabolic pathway. Process Biochem 43:1004–1008

    Article  CAS  Google Scholar 

  • Mallick S, Chatterjee S, Dutta TK (2007) A novel degradation pathway in the assimilation of phenanthrene by Staphylococcus sp. strain PN/Y via meta-cleavage of 2-hydroxy-1-naphthoic acid: formation of trans-2, 3-dioxo-5-(2′-hydroxyphenyl)-pent-4-enoic acid. Microbiology 153:2104–2115

    Article  CAS  Google Scholar 

  • Moody JD, Freeman JP, Doerge DR, Cerniglia CE (2001) Degradation of phenanthrene and anthracene by cell suspensions of Mycobacterium sp. strain PYR-1. Appl Environ Microbiol 67:1476

    Article  CAS  Google Scholar 

  • Parikh SJ, Chorover J, Burgos WD (2004) Interaction of phenanthrene and its primary metabolite (1-hydroxy-2-naphthoic acid) with estuarine sediments and humic fractions. J Contam Hydrol 72:1–22

    Article  CAS  Google Scholar 

  • Pasha C, Kuhad R, Rao LV (2007) Strain improvement of thermotolerant Saccharomyces cerevisiae VS3 strain for better utilization of lignocellulosic substrates. J Appl Microbiol 103:1480–1489

    Article  CAS  Google Scholar 

  • Peng RH, Xiong AS, Xue Y, Fu XY, Gao F, Zhao W, Tian YS, Yao QH (2008) Microbial biodegradation of polyaromatic hydrocarbons. FEMS Microbiol Rev 32:927–955

    Article  CAS  Google Scholar 

  • Pinyakong O, Habe H, Supaka N, Pinpanichkarn P, Juntongjin K, Yoshida T, Furihata K, Nojiri H, Yamane H, Omori T (2000) Identification of novel metabolites in the degradation of phenanthrene by Sphingomonas sp. strain P2. FEMS Microbiol Lett 191:115–121

    Article  CAS  Google Scholar 

  • Reddy MS, Naresh B, Leela T, Prashanthi M, Madhusudhan NC, Dhanasri G, Devi P (2010) Biodegradation of phenanthrene with biosurfactant production by a new strain of Brevibacillus sp. Bioresour Technol 101:7980–7983

    Article  CAS  Google Scholar 

  • Romero M, Cazau M, Giorgieri S, Arambarri A (1998) Phenanthrene degradation by microorganisms isolated from a contaminated stream. Environ Pollut 101:355–359

    Article  CAS  Google Scholar 

  • Samanta SK, Singh OV, Jain RK (2002) Polycyclic aromatic hydrocarbons: environmental pollution and bioremediation. Trends Biotechnol 20:243–248

    Article  CAS  Google Scholar 

  • Seo JS, Keum YS, Hu Y, Lee SE, Li QX (2006) Phenanthrene degradation in Arthrobacter sp. P1–1: initial 1, 2-, 3, 4- and 9, 10-dioxygenation, and meta- and ortho-cleavages of naphthalene-1, 2-diol after its formation from naphthalene-1, 2-dicarboxylic acid and hydroxyl naphthoic acids. Chemosphere 65:2388–2394

    Article  CAS  Google Scholar 

  • Seo JS, Keum YS, Hu Y, Lee SE, Li QX (2007) Degradation of phenanthrene by Burkholderia sp. C3: initial 1, 2- and 3, 4-dioxygenation and meta- and ortho-cleavage of naphthalene-1, 2-diol. Biodegradation 18:123–131

    Article  CAS  Google Scholar 

  • Tam N, Guo C, Yau W, Wong Y (2002) Preliminary study on biodegradation of phenanthrene by bacteria isolated from mangrove sediments in Hong Kong. Mar Pollut Bull 45:316–324

    Article  CAS  Google Scholar 

  • Tao XQ, Lu GN, Yi XY, Dang Z (2006) Isolation of phenanthrene-degrading microorganisms and analysis of metabolites of phenanthrene. J Agro-Environ Sci 25:190–195

    CAS  Google Scholar 

  • Tao XQ, Lu GN, Dang Z, Yang C, Yi XY (2007) A phenanthrene-degrading strain Sphingomonas sp. GY2B isolated from contaminated soils. Process Biochem 42:401–408

    Article  CAS  Google Scholar 

  • Tian L, Ma P, Zhong JJ (2002) Kinetics and key enzyme activities of phenanthrene degradation by Pseudomonas mendocina. Process Biochem 37:1431–1437

    Article  CAS  Google Scholar 

  • Ting W, Yuan S, Wu S, Chang B (2011) Biodegradation of phenanthrene and pyrene by ganoderma lucidum. Int Biodeterior Biodegradation 65:238–242

    Article  CAS  Google Scholar 

  • Tiwari JN, Reddy MMK, Patel DK, Jain SK, Murthy RC, Manickam N (2010) Isolation of pyrene degrading achromobacter xylooxidans and characterization of metabolic product. World J Microbiol Biotechnol 26:1727–1733

    Article  CAS  Google Scholar 

  • Wong JWC, Fang M, Zhao Z, Xing B (2004) Effect of surfactants on solubilization and degradation of phenanthrene under thermophilic conditions. J Environ Qual 33:2015–2025

    Article  CAS  Google Scholar 

  • Xue W, Warshawsky D (2005) Metabolic activation of polycyclic and heterocyclic aromatic hydrocarbons and DNA damage: a review. Toxicol Appl Pharmacol 206:73–93

    Article  CAS  Google Scholar 

  • Zhao HP, Wu QS, Wang L, Zhao XT, Gao HW (2009) Degradation of phenanthrene by bacterial strain isolated from soil in oil refinery fields in shanghai china. J Hazard Mater 164:863–869

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (No. 41101291 and 21007017), the Guangdong Provincial Natural Science Foundation (9351064101000001, S2012040007463 and S2012010008589), the Open Foundation of the Key Laboratory of Water and Air Pollution Control of Guangdong Province (2011A060901002), and the Science and Technology Planning Project of Guangzhou City (12C62081569).

Author information

Authors and Affiliations

  1. School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou, 510006, China

    Jing Lu, Chuling Guo, Jing Li, Hui Zhang, Guining Lu & Zhi Dang

  2. The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou, 510006, China

    Chuling Guo & Zhi Dang

  3. The Key Laboratory of Water and Air Pollution Control of Guangdong Province, Guangzhou, 510655, China

    Guining Lu & Renren Wu

Authors
  1. Jing Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chuling Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guining Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhi Dang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Renren Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chuling Guo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lu, J., Guo, C., Li, J. et al. A fusant of Sphingomonas sp. GY2B and Pseudomonas sp. GP3A with high capacity of degrading phenanthrene. World J Microbiol Biotechnol 29, 1685–1694 (2013). https://doi.org/10.1007/s11274-013-1331-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-013-1331-3

Keywords

Search

Navigation