World Journal of Microbiology and Biotechnology

, Volume 29, Issue 8, pp 1461–1471 | Cite as

Use of a glass bead-containing liquid medium for efficient production of a soil-free culture with polychlorinated biphenyl-dechlorination activity

  • Daisuke SuzukiEmail author
  • Daisuke Baba
  • Velayudhan Satheeja Santhi
  • Robinson David Jebakumar Solomon
  • Arata Katayama
Original Paper


We established a soil-free culture capable of dechlorinating polychlorinated biphenyls (PCBs) in Kanechlor-300 and Kanechlor-400 by establishing a PCB-dechlorinating soil culture in liquid medium containing 0.5 mm glass beads. PCB-dechlorination activity in liquid cultures with glass beads appeared to depend on the size of the glass beads, and soil-free cultures with 0.05-, 1.0- or 2.0 mm glass beads did not dechlorinate PCBs. Soil-free culture without glass beads also failed to dechlorinate PCBs. The soil-free culture containing 0.5 mm glass beads dechlorinated 42.6 ± 12.0 mol% in total PCBs. This soil-free culture was more effective than soil culture for dechlorinating PCBs ranging from dichlorinated PCBs to tetrachlorinated PCBs. Clone analysis of the 16S rRNA gene sequences showed that one of the predominant groups of microorganisms in the soil-free culture comprised heat-tolerant and spore-forming bacteria from the phylum Firmicutes. Heat treatment (100 °C, 10 min) did not destroy the PCB-dechlorination activity of the soil-free culture with glass beads. These results suggest that unknown species of the phylum Firmicutes were involved in PCB dechlorination in the soil-free culture. In this study, we succeeded in using a liquid medium containing glass beads as an inorganic soil substitute and showed that such a medium enhances PCB-dechlorination activity. Our study provides valuable information for developing PCB-bioremediation techniques using dechlorinating bacteria in anoxic contaminated soils and sediments.


Polychlorinated biphenyls Dechlorination Glass beads 16S rRNA gene 



This study was partially supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (23310055, 23658272).


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Daisuke Suzuki
    • 1
    Email author
  • Daisuke Baba
    • 1
  • Velayudhan Satheeja Santhi
    • 2
  • Robinson David Jebakumar Solomon
    • 2
  • Arata Katayama
    • 1
  1. 1.EcoTopia Science InstituteNagoya UniversityNagoyaJapan
  2. 2.Department of Molecular Microbiology, School of BiotechnologyMadurai Kamaraj UniversityMaduraiIndia

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