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Enhancing adhesion of yeast brewery strains to chamotte carriers through aminosilane surface modification

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Abstract

The adhesion of cells to solid supports is described as surface-dependent, being largely determined by the properties of the surface. In this study, ceramic surfaces modified using different organosilanes were tested for proadhesive properties using industrial brewery yeast strains in different physiological states. Eight brewing strains were tested: bottom-fermenting Saccharomyces pastorianus and top-fermenting Saccharomyces cerevisiae. To determine adhesion efficiency light microscopy, scanning electron microscopy and the fluorymetric method were used. Modification of chamotte carriers by 3-(3-anino-2-hydroxy-1-propoxy) propyldimethoxysilane and 3-(N, N-dimethyl-N-2-hydroxyethyl) ammonium propyldimethoxysilane groups increased their biomass load significantly.

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Acknowledgments

This work was supported by the Ministry of Scientific Research and Information Technology, Grants No N312 018 32/1283 and N205 129 935. We would like to give our heartfelt thanks to Urszula Mizerska and Witold Fortuniak (the Centre of Molecular and Macromolecular Studies, the Polish Academy of Science) for the preparation of the modified carriers.

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Authors and Affiliations

  1. Institute of Fermentation Technology and Microbiology, Technical University of Lodz, ul. Wolczanska 171/173, 90-924, Lodz, Poland

    Joanna Berlowska, Dorota Kregiel & Wojciech Ambroziak

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  1. Joanna Berlowska
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  2. Dorota Kregiel
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  3. Wojciech Ambroziak
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Correspondence to Joanna Berlowska.

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Berlowska, J., Kregiel, D. & Ambroziak, W. Enhancing adhesion of yeast brewery strains to chamotte carriers through aminosilane surface modification. World J Microbiol Biotechnol 29, 1307–1316 (2013). https://doi.org/10.1007/s11274-013-1294-4

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  • DOI: https://doi.org/10.1007/s11274-013-1294-4

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