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Potato signal molecules that activate pectate lyase synthesis in Pectobacterium atrosepticum SCRI1043

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Abstract

A new type of plant-derived signal molecules that activate extracellular pectate lyase activity in phytopathogenic bacterium Pectobacterium atrosepticum SCRI1043 was revealed. These compounds were characterized and partially purified by means of several approaches including RT-PCR analysis, luminescence bioassay and HPLC fractionation. They were smaller than 1 kDa, thermoresistant, nonproteinaceous, hydrophilic, and slightly negatively charged molecules. Using gene expression analysis and bacterial biosensor assay the mode of activity of revealed compounds was studied. The possibility of their action through quorum sensing- and KdgR-mediated pathways was analyzed.

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Acknowledgments

This work was supported in part by the Russian Foundation for Basic Research, research project No. 12-04-31059-MOL A and by a grant from the programme “Leading Scientific Schools” No. NSH-825.2012.4 (Supervisor A.N. Grechkin).

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Authors and Affiliations

  1. Kazan Research Center, Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, PO Box 30, Kazan, 420111, Russia

    Nadezhda Tarasova, Vladimir Gorshkov, Olga Petrova & Yuri Gogolev

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  1. Nadezhda Tarasova
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  2. Vladimir Gorshkov
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  3. Olga Petrova
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  4. Yuri Gogolev
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Correspondence to Nadezhda Tarasova.

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Tarasova, N., Gorshkov, V., Petrova, O. et al. Potato signal molecules that activate pectate lyase synthesis in Pectobacterium atrosepticum SCRI1043. World J Microbiol Biotechnol 29, 1189–1196 (2013). https://doi.org/10.1007/s11274-013-1281-9

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  • DOI: https://doi.org/10.1007/s11274-013-1281-9

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