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Potential application of aromatic plant extracts to prevent cheese blowing

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Abstract

This study aimed to inhibit the growth of Escherichia coli and Clostridium tyrobutyricum, common bacteria responsible for early and late cheese blowing defects respectively, by using novel aqueous extracts obtained by dynamic solid–liquid extraction and essential oils obtained by solvent free microwave extraction from 12 aromatic plants. In terms of antibacterial activity, a total of 13 extracts inhibited one of the two bacteria, and only two essential oils, Lavandula angustifolia Mill. and Lavandula hybrida, inhibited both. Four aqueous extracts were capable of inhibiting C. tyrobutyricum, but none were effective against E. coli. After extracts’ chemical composition identification, relationship between the identified compounds and their antibacterial activity were performed by partial least square regression models revealing that compounds such as 1,8 cineole, linalool, linalyl acetate, β-phellandrene or verbene (present in essential oils), pinocarvone, pinocamphone or coumaric acid derivate (in aqueous extracts) were compounds highly correlated to the antibacterial activity.

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Acknowledgments

The research leading to these results has received funding from the European Union’s Seventh Framework Programme managed by REA-Research Executive Agency http://ec.europa.eu/research/rea (FP7/2007-2013) under grant agreement n FP7-SME-2008-2-243638-TECHeese. We wish to thank the entire Project Consortium, along with Ms. Ryniak, the Project Officer, for their support and collaboration. Thanks also go to S. Lozoya and J.A. Laborda, for their technical support with the plants extraction and the antibacterial assay.

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Authors and Affiliations

  1. Departamento de Ciencia y Tecnología Agroforestal y Genética, E.T.S.I.A-I.D.R., Universidad de Castilla-La Mancha, Campus Universitario 02071, Albacete, Spain

    C. M. Librán, A. Molina & M. I. Berruga

  2. Cátedra de Química Agrícola, E.T.S.I.A., Universidad de Castilla-La Mancha, Campus Universitario 02071, Albacete, Spain

    A. Moro & A. Zalacain

  3. Parque Científico y Tecnológico de Albacete, Campus Universitario 02071, Albacete, Spain

    M. Carmona

Authors
  1. C. M. Librán
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  2. A. Moro
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  3. A. Zalacain
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  4. A. Molina
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  5. M. Carmona
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  6. M. I. Berruga
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Corresponding author

Correspondence to M. I. Berruga.

Additional information

C. M. Librán and A. Moro have contributed equally to this work.

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Librán, C.M., Moro, A., Zalacain, A. et al. Potential application of aromatic plant extracts to prevent cheese blowing. World J Microbiol Biotechnol 29, 1179–1188 (2013). https://doi.org/10.1007/s11274-013-1280-x

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  • DOI: https://doi.org/10.1007/s11274-013-1280-x

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