Abstract
The aim of this study was to survey microorganisms from the deep surface sediment samples collected from the Sea of Japan and to screen them for antimicrobial and antagonistic effects. Phylogenetic analysis revealed most isolates sharing 98–100 % sequence similarity to recognized species, including those recovered previously from marine or saline environments. Alteromonas, Halomonas, Marinobacter, Pseudoalteromonas, Salinicola, within the class Gammaproteobacteria, Sulfitobacter (Alphaproteobacteria), Bacillus, Paenibacillus and Paenisporosarcina (Firmicutes), Nocardiopsis and Streptomyces (Actinobacteria) occurred abundantly in all sediment samples. Antimicrobial screening revealed twenty three strains (13 %) capable to inhibit growth of one to eight test cultures and deep sediment isolates. Based on phylogenetic analysis mostly active strains belonged to the genera Bacillus, Brevibacillus, Nocardiopsis, Paenibacillus and Streptomyces. Antimicrobial substances (1–3) were isolated from strain Paenibacillus sp. Sl 79w showing a high inhibitory activity. On the basis of combined spectral analyses (IR, UV, 1H and 13C NMR) the compounds 1, 2 and 3 with [M + H]+ at 409.1 and 409.2 m/z, and with [M + Na]+ at 822.5 m/z were found to have a carbon skeleton of isocoumarin and peptide antibiotics, respectively. Our findings demonstrated that the deep surface sediments of the Sea of Japan represent an untapped source of diverse microorganisms capable of antimicrobial metabolite production.
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Acknowledgments
We thank all participants of Russian-German deep-sea expedition (SoJaBio), R/V Akademik Lavrentyev 51st Cruise, for providing the sediment samples; to thank Dr. Claudine Vereecke, BCCM™/LMG Bacteria Collection, Ghent University, Gent, Belgium, and Dr. Chantal Bizet, the Collection de l’Institut Pasteur, Institut Pasteur, CIP, Paris, France, for providing indicator strains. This study was supported by a grant no. 11-04-98538 from the Russian Foundation for Basic Research (RFBR) and Far-Eastern Branch of Russian Academy of Sciences, by a grant no. 12-III-A-06-105 from the Presidium of Far-Eastern Branch of Russian Academy of Sciences and by a grant no. 11-04-00781-a from the RFBR.
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Romanenko, L.A., Tanaka, N., Kalinovskaya, N.I. et al. Antimicrobial potential of deep surface sediment associated bacteria from the Sea of Japan. World J Microbiol Biotechnol 29, 1169–1177 (2013). https://doi.org/10.1007/s11274-013-1276-6
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DOI: https://doi.org/10.1007/s11274-013-1276-6