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Molecular and pathogenic characterization of new Xanthomonas oryzae pv. oryzae strains from the coastline region of Fangchenggang city in China

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Abstract

Virulence assays and DNA polymorphism analyses were used to characterize 33 Xanthomonas oryzae pv. oryzae (Xoo) strains collected from the coastline region of Fangchenggang city in China. Two new pathogenic races (FXP1 and FXP2), were determined by leaf-clipping inoculation of 12 near-isogenic International Rice-Bacterial Blight (IRBB) rice lines, each containing a single resistance gene. Race FXP1 consisted of twenty-eight strains that were incompatible on IRBB5 and IRBB7, while race FXP2 included five strains that were incompatible on IRBB5 and IRBB7 and moderately virulent on IRBB8 containing the xa8 gene. Restriction fragment length polymorphism (RFLP) analysis revealed that each probe of avrXa10 and IS1112 resolved two haplotypes. In a dendrogram generated from the combined RFLP data, the 33 Xoo strains were resolved into two clusters. There was a weak correlation (r = 0.53) between race and haplotype. All of the rice cultivars planted in the coastline region of Fangchenggang city were susceptible to the representative Xoo strains tested above. However, we found that four rice cultivars used as breeding materials in the laboratory could fully resist infection by the Xoo strains, suggesting that the isolated Xoo strains could be used to detect resistant rice cultivars suitable for planting in the local rice field.

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Acknowledgments

This work was supported by the 973 Program of the Ministry of Science and Technology of China (2012CB114003) and the Bagui Scholar Program of Guangxi (2011A001). We thank the Plant Protection Station of Fangchenggang city for assistance in collecting rice leaves having BLB. We thank the International Rice Research Institute (IRRI) for rice seeds of IR24 and IRBB lines. We thank Dr. Valerie Verdier at IRD, France for providing biological materials and helpful discussions.

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530004, China

    Shu-Qing Yang, Shu-Yan Liu, Shuai Zhao, Yan-Hua Yu, Cheng-Jie Duan, Ji-Liang Tang & Jia-Xun Feng

  2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agronomy, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530004, China

    Rong-Bai Li

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  1. Shu-Qing Yang
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  2. Shu-Yan Liu
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  3. Shuai Zhao
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  4. Yan-Hua Yu
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  5. Rong-Bai Li
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Correspondence to Jia-Xun Feng.

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Shu-Qing Yang, Shu-Yan Liu and Shuai Zhao contributed equally to this study.

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Yang, SQ., Liu, SY., Zhao, S. et al. Molecular and pathogenic characterization of new Xanthomonas oryzae pv. oryzae strains from the coastline region of Fangchenggang city in China. World J Microbiol Biotechnol 29, 713–720 (2013). https://doi.org/10.1007/s11274-012-1227-7

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