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Ethanol production by Saccharomyces cerevisiae using lignocellulosic hydrolysate from Chrysanthemum waste degradation

World Journal of Microbiology and Biotechnology volume 29, pages 459–466 (2013)Cite this article

Abstract

Ethanol production derived from Saccharomyces cerevisiae fermentation of a hydrolysate from floriculture waste degradation was studied. The hydrolysate was produced from Chrysanthemum (Dendranthema grandiflora) waste degradation by Pleurotus ostreatus and characterized to determine the presence of compounds that may inhibit fermentation. The products of hydrolysis confirmed by HPLC were cellobiose, glucose, xylose and mannose. The hydrolysate was fermented by S. cerevisiae, and concentrations of biomass, ethanol, and glucose were determined as a function of time. Results were compared to YGC modified medium (yeast extract, glucose and chloramphenicol) fermentation. Ethanol yield was 0.45 g g−1, 88 % of the maximal theoretical value. Crysanthemum waste hydrolysate was suitable for ethanol production, containing glucose and mannose with adequate nutrients for S. cerevisiae fermentation and low fermentation inhibitor levels.

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Acknowledgments

The authors acknowledge financial support from Pontificia Universidad Javeriana and the División de Investigación de la Sede Bogotá (DIB) at Universidad Nacional de Colombia. We also thank Cultivos del Norte for providing the Chrysanthemum waste and Maria Lucia Gutierrez for English editing.

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Affiliations

  1. Grupo de Biotecnología Ambiental e Industrial (GBAI), Laboratorio de Biotecnología Aplicada, Departamento de Microbiología, Pontificia Universidad Javeriana, Bogotá, Colombia

    Balkys Quevedo-Hidalgo

  2. Carrera 7 No 43-68 oficina 618, edificio 52, Bogotá, Colombia

    Balkys Quevedo-Hidalgo

  3. Industrial Microbiologist, Pontificia Universidad Javeriana, Bogotá, Colombia

    Felipe Monsalve-Marín

  4. Grupo de Procesos Químicos y Bioquímicos, Laboratorio de Ingeniería Química, Departamento de Ingeniería Química y Ambiental, Universidad Nacional de Colombia sede Bogotá, Carrera 30 No 45-03, edificio 412, Bogotá, Colombia

    Paulo César Narváez-Rincón & Mario Enrique Velásquez-Lozano

  5. Grupo de Biotecnología Ambiental e Industrial (GBAI), Laboratorio de Microbiología ambiental, Departamento de Microbiología, Pontificia Universidad Javeriana, Carrera 7 No 43-68 oficina 618, edificio 52, Bogotá, Colombia

    Aura Marina Pedroza-Rodríguez

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  1. Balkys Quevedo-Hidalgo
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  2. Felipe Monsalve-Marín
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  3. Paulo César Narváez-Rincón
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  4. Aura Marina Pedroza-Rodríguez
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  5. Mario Enrique Velásquez-Lozano
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Correspondence to Balkys Quevedo-Hidalgo.

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Quevedo-Hidalgo, B., Monsalve-Marín, F., Narváez-Rincón, P.C. et al. Ethanol production by Saccharomyces cerevisiae using lignocellulosic hydrolysate from Chrysanthemum waste degradation. World J Microbiol Biotechnol 29, 459–466 (2013). https://doi.org/10.1007/s11274-012-1199-7

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  • DOI: https://doi.org/10.1007/s11274-012-1199-7

Keywords

  • Ethanol
  • Saccharomyces cerevisiae
  • Chrysanthemum
  • Lignocellulose