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Effects of critical medium components on the production of antifungal lipopeptides from Bacillus amyloliquefaciens Q-426 exhibiting excellent biosurfactant properties

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Abstract

In this study, influence of three critical parameters nitrogen sources, initial pH and metal ions was discussed in the production of antifungal lipopeptides from Bacillus amyloliquefaciens Q-426. The results revealed that lipopeptide biosynthesis might have relations with the population density of strain Q-426 and some special amino acids. Also, the alkali-resistant strain Q-426 could grow well in the presence of Fe2+ ions below 0.8 M l−1 and still maintain the competitive advantage below 0.2 M l−1. Moreover, lipopeptides exhibited significant inhibitory activities against Curvularia lunata (Walk) Boed even at the extreme conditions of temperature, pH and salinity. Finally, biosurfactant properties of lipopeptides mixture were evaluated by use with totally six different methods including bacterial adhesion to hydrocarbons assay, lipase activity, hemolytic activity, emulsification activity, oil displacement test and surface tension measurement. The research suggested that B. amyloliquefaciens Q-426 may have great potential in agricultural and environmental fields.

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Acknowledgments

This work was supported by the State Ethic Affairs Commission, the Minister of Education (SEAC-ME) and the Key Laboratory Foundation of Liaoning Provincial at the Department of Education, China. Especially, we thanked to Dr. Jing Zhao (Dalian Nationalities University) for revising the whole paper carefully.

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Authors and Affiliations

  1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhong-shan Road, Dalian, 116023, China

    Pengchao Zhao & Lina Wang

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100049, China

    Pengchao Zhao & Lina Wang

  3. Department of Life Science, Dalian Nationalities University, Economical and Technological Development Zone, Dalian, 116600, China

    Chunshan Quan, Liming Jin & Shengdi Fan

  4. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China

    Jianhua Wang

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  1. Pengchao Zhao
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  2. Chunshan Quan
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  3. Liming Jin
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  4. Lina Wang
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  5. Jianhua Wang
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  6. Shengdi Fan
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Correspondence to Chunshan Quan.

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Pengchao Zhao, Chunshan Quan: Equal contribution as first authors.

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Zhao, P., Quan, C., Jin, L. et al. Effects of critical medium components on the production of antifungal lipopeptides from Bacillus amyloliquefaciens Q-426 exhibiting excellent biosurfactant properties. World J Microbiol Biotechnol 29, 401–409 (2013). https://doi.org/10.1007/s11274-012-1180-5

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  • DOI: https://doi.org/10.1007/s11274-012-1180-5

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