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World Journal of Microbiology and Biotechnology

, Volume 29, Issue 2, pp 257–265 | Cite as

Probiotics in the intestinal tract of juvenile whiteleg shrimp Litopenaeus vannamei: modulation of the bacterial community

  • Irasema E. Luis-Villaseñor
  • Thelma Castellanos-Cervantes
  • Bruno Gomez-Gil
  • Ángel E. Carrillo-García
  • Ángel I. Campa-Córdova
  • Felipe AscencioEmail author
Original Paper

Abstract

Molecular analysis of the 16S rDNA of the intestinal microbiota of whiteleg shrimp Litopenaeus vannamei was examined to investigate the effect of a Bacillus mix (Bacillus endophyticus YC3-b, Bacillus endophyticus C2-2, Bacillus tequilensisYC5-2) and the commercial probiotic (Alibio®) on intestinal bacterial communities and resistance to Vibrio infection. PCR and single strain conformation polymorphism (SSCP) analyses were then performed on DNA extracted directly from guts. Injection of shrimp with V. parahaemolyticus at 2.5 × 105 CFU g−1 per shrimp followed 168 h after inoculation with Bacillus mix or the Alibio probiotic or the positive control. Diversity analyses showed that the bacterial community resulting from the Bacillus mix had the highest diversity and evenness and the bacterial community of the control had the lowest diversity. The bacterial community treated with probiotics mainly consisted of α- and γ-proteobacteria, fusobacteria, sphingobacteria, and flavobacteria, while the control mainly consisted of α-proteobacteria and flavobacteria. Differences were grouped using principal component analyses of PCR-SSCP of the microbiota, according to the time of inoculation. In Vibrio parahaemolyticus-infected shrimp, the Bacillus mix (~33 %) induced a significant increase in survival compared to Alibio (~21 %) and the control (~9 %). We conclude that administration of the Bacillus mix induced modulation of the intestinal microbiota of L. vannamei and increased its resistance to V. parahaemolyticus.

Keywords

Whiteleg shrimp Bacteria community Probiotics Bacillus mix 

Notes

Acknowledgments

The authors thank Maria de Jésus Romero Geraldo of CIBNOR for assistance. Ira Fogel of CIBNOR provided editorial services. The project was funded by Consejo Nacional de Ciencia y Tecnología, SEP-CONACYT grant 25981, and Centro de Investigaciones Biológicas del Noroeste grant AC0.4. I.E.L.V. is a recipient of a CONACYT fellowship (grant 48351).

Supplementary material

11274_2012_1177_MOESM1_ESM.docx (29 kb)
Supplementary material 1 (DOCX 29 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Irasema E. Luis-Villaseñor
    • 1
  • Thelma Castellanos-Cervantes
    • 1
  • Bruno Gomez-Gil
    • 2
  • Ángel E. Carrillo-García
    • 1
  • Ángel I. Campa-Córdova
    • 1
  • Felipe Ascencio
    • 1
    Email author
  1. 1.Centro de Investigaciones Biológicas del Noroeste (CIBNOR)Instituto Politécnico Nacional 195La PazMexico
  2. 2.Center for Research in Nutrition and Development (CIAD)Mazatlan Unit for AquacultureSinaloaMexico

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