Manufacture of Cheddar cheese using probiotic Lactobacillus plantarum K25 and its cholesterol-lowering effects in a mice model

Abstract

The probiotic adjunct Lactobacillus plantarum K25 was inoculated into milk to produce probiotic cheese. The effect of Lb. plantarum K25 on cheese composition, microbiological growth and survival during the manufacturing and ripening period, primary and secondary proteolysis during cheese ripening, and the in vivo cholesterol-lowering ability of the probiotic cheese were investigated. The results showed that the use of adjunct Lb. plantarum K25 in Cheddar cheese did not affect the cheese components including moisture, protein, fat, salt content and the pH value of cheese. During the whole ripening period, the probiotic adjunct maintained its viability, suggesting the effectiveness of Cheddar cheese as a vehicle for delivery of probiotic bacteria. No significant differences were observed in water-soluble nitrogen, 70 % ethanol-soluble nitrogen, 5 % phosphotungstic acid-soluble nitrogen, free amino acids and urea-PAGE patterns between the control and probiotic cheeses. Assessment of the in vivo cholesterol-lowering property of cheese with Lb. plantarum K25 showed that the levels of serum total cholesterol, low-density lipoprotein cholesterol and triglycerides decreased significantly, and the level of serum high-density lipoprotein cholesterol increased in mice fed with the probiotic cheese. The results indicated the potential function as a dietary item of the probiotic cheese with Lb. plantarum K25 to reduce the risk of cardiovascular diseases.

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Acknowledgments

The financial support for this work from The Earmarked Fund for Modern Agro-industrial Technology Research Systems in China (CARS-37), National Public Benefit Research (Agriculture) Foundation (200903043), Natural Science Foundation for the Youth (31101245), and the Foundation for Youth of Jilin Province (201101126) is gratefully acknowledged.

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Correspondence to Zhennai Yang.

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Zhang, L., Zhang, X., Liu, C. et al. Manufacture of Cheddar cheese using probiotic Lactobacillus plantarum K25 and its cholesterol-lowering effects in a mice model. World J Microbiol Biotechnol 29, 127–135 (2013). https://doi.org/10.1007/s11274-012-1165-4

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Keywords

  • Cheddar cheese
  • Lactobacillus plantarum
  • Probiotic adjuncts
  • Proteolysis
  • Cholesterol-lowering ability