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Purification and characterization of an extracellular poly(3-hydroxybutyrate-co-3-hydroxyvalerate) depolymerase from Acidovorax sp. HB01

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Abstract

The poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)-degrading strain Acidovorax sp. HB01 was isolated from an activated sludge sample. A novel PHBV depolymerase with a molecular weight of 43.4 kDa was purified to homogeneity from the culture supernatant of the HB01 strain. The optimum pH and temperature of the PHBV depolymerase were 7.0 and 50 °C, respectively. The PHBV depolymerase can also degrade polyhydroxybutyrate, poly (3-hydroxybutyrate-co-4-hydroxybutyrate), and poly(caprolactone); however, the PHBV degradation activity of the depolymerase is higher than its activity against the other polymers. Effect of metal ions and various inhibitors on the PHBV depolymerase activity was examined. The addition of Na+, K+, and Ca2+ markedly increased the hydrolysis rate, whereas the enzyme activity was inhibited by Zn2+, Mg2+, Mn2+, and particularly by Cu2+ and Fe2+. Ethylenediaminetetraacetic acid was found to have a significant inhibitory effect. The main degradation product of depolymerase was identified as the 3-hydroxybutyric acid monomer and 3-hydroxyvaleric acid monomers via mass spectrometry.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 31100099) and Science Foundation of Liaoning Shihua University (No. 2011XJJ-025).

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  1. School of Environmental and Biological Engineering, Liaoning Shihua University, Fushun, 113001, China

    Zhanyong Wang, Jia Gao, Linlin Li & Husheng Jiang

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  1. Zhanyong Wang
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  2. Jia Gao
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  3. Linlin Li
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  4. Husheng Jiang
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Correspondence to Zhanyong Wang.

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Wang, Z., Gao, J., Li, L. et al. Purification and characterization of an extracellular poly(3-hydroxybutyrate-co-3-hydroxyvalerate) depolymerase from Acidovorax sp. HB01. World J Microbiol Biotechnol 28, 2395–2402 (2012). https://doi.org/10.1007/s11274-012-1048-8

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  • DOI: https://doi.org/10.1007/s11274-012-1048-8

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