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Bioactive secondary metabolites from Nigrospora sp. LLGLM003, an endophytic fungus of the medicinal plant Moringa oleifera Lam.

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Abstract

An endophytic fungus was isolated from the root of the medicinal plant Moringa oleifera Lam. Based on analyzing the rDNA sequence, the fungus was identified as Nigrospora sp. This is the first report of the isolation of endophytic Nigrospora from M. oleifera. By bioassay-guided fractionation, four antifungal secondary metabolites were isolated from liquid cultures of the fungus Nigrospora sp. LLGLM003, and their chemical structures were determined to be griseofulvin (1), dechlorogriseofulvin (2), 8-dihydroramulosin (3) and mellein (4) on the basis of spectroscopic analyses. Compound 2, 3 and 4 were isolated from Nigrospora sp. for the first time. In vitro antifungal assay showed that griseofulvin displayed clear inhibition of the growth of 8 plant pathogenic fungi. Dechlorogriseofulvin and mellein exhibited only weak antifungal activities, whereas 8-dihydroramulosin displayed no antifungal activities.

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Acknowledgments

This work was supported by Special Fund for Agro-scientific Research in the Public Interest (200903052) to Chu-long Zhang.

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Authors and Affiliations

  1. College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310014, People’s Republic of China

    J. H. Zhao & Y. L. Zhang

  2. Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    J. Y. Wang & C. L. Zhang

  3. Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    J. H. Zhao

  4. Department of Pharmaceutical Science, College of Medicine and Public Health Management, Hangzhou Normal University, Hangzhou, 310036, People’s Republic of China

    L. W. Wang

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  1. J. H. Zhao
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Correspondence to C. L. Zhang.

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Zhao, J.H., Zhang, Y.L., Wang, L.W. et al. Bioactive secondary metabolites from Nigrospora sp. LLGLM003, an endophytic fungus of the medicinal plant Moringa oleifera Lam.. World J Microbiol Biotechnol 28, 2107–2112 (2012). https://doi.org/10.1007/s11274-012-1015-4

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  • DOI: https://doi.org/10.1007/s11274-012-1015-4

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