Skip to main content
SpringerLink
Log in

Effect of chemical and microbial vitamin B12 analogues on production of vitamin B12

  • Short Communication
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Strain improvement by genetic manipulation or optimization of fermentation conditions for overproduction of vitamin B12 has a drawback due to feed back inhibition. To resist the feed back inhibition by analogues of vitamin B12 in Propionibacterium freudenrechii subsps. shermanii (OLP-5), we have tested with microbially separated B12 analogues from three different strains. Microbial analogues were differentiated from commercially available vitamin B12 by high pressure liquid chromatography and spectrophotometric method. An analogue isolated from NRRL-B-4327 was shown to increase vitamin B12 concentration from 18.53 ± 0.15 to 31.67 ± 0.58 mg/l in OLP-5 strain. The presence of chemical analogue (ICH2 Co(DH)2 (H2Py)4) increased vitamin B12 production from 16.13 ± 0.15 to 18.53 ± 0.15 mg/l in OLP-5. These findings revealed that addition of B12 analogues in fermentation media have developed strain resistance to feed back inhibition by vitamin B12.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References:

  • Gardner N, Champagne CP (2005) Production of Propionibacterium Shermanii biomass and vitamin B12 on spent media. J Appl Microbiol 99:1236–1245

    Article  CAS  Google Scholar 

  • Girard CL, Santschi DE, Stabler SP, Allen RH (2009) Apparent ruminal synthesis and intestinal disappearance of vitamin B12 and its analogs in dairy cows. J Dairy Sci 92:4524–4529

    Article  CAS  Google Scholar 

  • Hugenschmidt S, Schwenninger SM, Lacroix C (2011) Concurrent high production of natural folate and vitamin B12 using a co-culture process with Lactobacillus plantarum SM39 and Propionibacterium freudenreichii DF13. Process Biochem 46(2011):1063–1070

    Article  CAS  Google Scholar 

  • Jan G, Leverrier P, Roland N (2002) Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations. Lait 82:131–144

    Article  CAS  Google Scholar 

  • Krfilovfi B, Rauch P (1985) The effect of antimetabolites on the microbial biosynthesis of vitamin B12 and related compounds. Appl Microbiol Biotechnol 21:331–335

    Google Scholar 

  • Lawrence J, Brandt MD, Lawrence Goldberg MD, Leslie H, Bernstein MD, Gerald Greenberg BS (1979) The effect of bacterially produced vitamin B12 analogues (cobamides) on the in vitro absorption of cyanocobalamin. Am J Clin Nutr 32:1832–1836

    Google Scholar 

  • Martens H, Barg H, Warren MJ, Jahn D (2002) Microbial production of vitamin B12. Appl Environ Microbiol 58:275–285

    CAS  Google Scholar 

  • Piao Y, Yamashita M, Kawaraichi N, Asegawa R, Ono H, Murooka Y (2004) Production of vitamin B12 in genetically engineered Propionibacterium freudenreichii. J Biosci Bioeng 98:167–173

    CAS  Google Scholar 

  • Quesada-Chanto A, Schmid-Meyer AC, Schroeder AG, Fuchter A, Carvalho-Jonas MF, Koehntopp PI, Jonas R (1998) Comparison of methods for determination of vitamin B12 in microbial material. Biotechnol Tech 12:75–77

    Article  CAS  Google Scholar 

  • Rebekka B, Marco M, Heiko B, Boyke B, Jan HM, Evelyne D, Helen L, Martin J, Warren I, Dieter J (2010) Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microb Biotecchnol 3(1):24–37

    Article  Google Scholar 

  • Robert W, Hansjorg T, Niklaus G, Schaefer, Dave R, van S, Stefan M, Susanne K, Martin K, Roger A, Jakob N, Alexander K, Holger M, Roger S, Schubiger PA (2008) New derivatives of vitamin B12 show preferential targeting of tumors. Cancer Res 68(8)

  • Rudking GO, Taylor RJ (1952) Chemical method for determining vitamin B12. Anal Chem 24:1155–1156

    Google Scholar 

  • Scott AI, Stolowich NJ, Wang J, Gawatz O, Fridrich E, Muller G (1996) Biosynthesis of vitamin B12: factor IV, a new intermediate in the anaerobic pathway. Proc Natl Acad Sci USA 93:14316–14319

    Article  CAS  Google Scholar 

  • Yang Y, Zhang Z, Lu J, Mackawa T (2004) Continuous methane production and the production of vitamin B12 in a fixed bed reactor. Bioresour Technol 92:285–290

    Article  CAS  Google Scholar 

  • Wyk JV, Britz TJ (2010) A rapid HPLC method for the extraction an quantification of vitamin B12 in dairy products and cultures of Propionibacterium freudenreichii. Dairy Sci Technol 90:509–520

    Google Scholar 

Download references

Acknowledgments

We are grateful to Department of Biotechnology, Govt. of India for providing the financial assistance and Department of Chemistry, Osmania University for providing us chemically synthesized analogues.

Author information

Authors and Affiliations

  1. Department of Microbiology, Osmania University, Hyderabad, 500007, Andhra Pradesh, India

    Yeruva Thirupathaiah, Chiliveri Swarupa Rani, Marrivada Sudhakara Reddy & Linga Venkateswar Rao

Authors
  1. Yeruva Thirupathaiah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chiliveri Swarupa Rani
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marrivada Sudhakara Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Linga Venkateswar Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Linga Venkateswar Rao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thirupathaiah, Y., Swarupa Rani, C., Sudhakara Reddy, M. et al. Effect of chemical and microbial vitamin B12 analogues on production of vitamin B12 . World J Microbiol Biotechnol 28, 2267–2271 (2012). https://doi.org/10.1007/s11274-012-1011-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-012-1011-8

Keywords

Search

Navigation