World Journal of Microbiology and Biotechnology

, Volume 28, Issue 3, pp 1107–1113 | Cite as

Identification of genes related to nitrogen uptake in wine strains of Saccharomyces cerevisiae

  • A. Contreras
  • V. García
  • F. Salinas
  • U. Urzúa
  • M. A. Ganga
  • C. MartínezEmail author
Original Paper


The yeast Saccharomyces cerevisiae is the main microorganism responsible for wine fermentation and its development influences the quality of wine. A problem affecting these types of fermentations, generating important losses in this industry, are the slow or stuck fermentations which may result from low nitrogen availability in the must. Therefore, several studies have been directed towards identifying genes involved in nitrogen metabolism using high throughput strategies which include subjecting the yeast to changes in the type or concentration of the available nitrogen source. However, this type of approach can also generate responses in the yeast that do not necessarily alter the expression of genes related to nitrogen metabolism. In this work, by using intraspecific hybridisation of wild wine yeast strains we obtained genetically and oenologically similar strains with differences in the consumption of nitrogen sources. Using the same must, the global expression patterns of these yeasts were compared by microarrays, the analysis of which identified 276 genes that varied in their expression between the strains analysed. The functional analysis of the genes with a known function indicates that some participate in nitrogen metabolism, alcoholic fermentation, ion transport and transcriptional regulation. Furthermore, differences were observed in the expression of genes which have been partially associated to nitrogen, as in the case of ZRT1 and ATO2. Interestingly, many of the genes identified have no known function or have not been previously associated to this phenotype.


Fermentation Nitrogen uptake Transcription analyses Wine Yeast 



This research was supported by grants 1100509 (Fondecyt) and AT 24091033 (Conicyt). V. Garcia was supported by a DICYT-USACH Postdoctoral Fellowship.

Supplementary material

11274_2011_911_MOESM1_ESM.xls (1.5 mb)
Supplementary material 1 (XLS 1570 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • A. Contreras
    • 1
  • V. García
    • 1
  • F. Salinas
    • 1
  • U. Urzúa
    • 3
  • M. A. Ganga
    • 1
  • C. Martínez
    • 1
    • 2
    Email author
  1. 1.Departamento de Ciencia y Tecnología de los AlimentosUniversidad de Santiago de Chile (USACH)SantiagoChile
  2. 2.Centro de Estudios en Ciencia y Tecnología de Alimentos (CECTA)Universidad de Santiago de Chile (USACH)SantiagoChile
  3. 3.Laboratorio de Genómica Aplicada, Programa de Biología Celular y Molecular-ICBMUniversidad de ChileSantiagoChile

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