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Global transcriptional analysis of stress-response strategies in Acidithiobacillus ferrooxidans ATCC 23270 exposed to organic extractant—Lix984n

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Abstract

Acidithiobacillus ferrooxidans (A. ferrooxidans) ATCC 23270 is a model bacteria for bioleaching research. Because of the use of extractant in metal extraction industry, A. ferrooxidans needs to cope with the water-organic two-phase system. To get insight into the molecular response of A. ferrooxidans to organic solvent, global gene expression pattern was examined in A. ferrooxidans ATCC 23270 cells subjected to Lix984n (an organic extractant) using the method of whole-genome DNA microarray. The data suggested that the global response of A. ferrooxidans to Lix984n stress was characterized by the up-regulation of genes involved in pentose phosphate pathway, fatty acid and glutamate biosynthesis. In further study, compared to heterotrophic bacteria in dealing with short-time stress, A. ferrooxidans has a special strategy of continuously enhancing the expression of genes encoding proteins involved in electron transport, such as petI, petII, cyo and cyd. Besides, acrAB-tolC operon encoding organic solvent efflux pump and its positive regulator gene ostR were addressed.

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Acknowledgments

We thank Ming Xie, Xian Fu and Xiangru Fan for participating in revising the manuscript. This research was supported by the National Natural Science Foundation of China (No. 50621063, No. 31070104, No. 30900203), Doctoral Fund of Ministry of Education of China No. 200805330035), New Teacher Fund for Doctor Station, the Ministry of Education (No. 200805331078) and the National Basic Research Program (No. 2010CB630901).

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Authors and Affiliations

  1. School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan, 410083, China

    Zhijun Zhou, Yun Fang, Huaqun Yin, Wenqing Qin, Yili Liang, Qian Li, Nuo Li, Xinxing Liu, Guanzhou Qiu & Xueduan Liu

  2. Key Laboratory of Biometallurgy of Ministry of Education, Changsha, Hunan, 410083, China

    Zhijun Zhou, Yun Fang, Huaqun Yin, Wenqing Qin, Yili Liang, Qian Li, Nuo Li, Xinxing Liu, Guanzhou Qiu & Xueduan Liu

  3. School of Metallurgical Science and Engineering, Central South University, Changsha, Hunan, 410083, China

    Qihou Li

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  1. Zhijun Zhou
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Correspondence to Huaqun Yin or Xueduan Liu.

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These authors Zhijun Zhou and Yun Fang contributed equally to this work.

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Zhou, Z., Fang, Y., Li, Q. et al. Global transcriptional analysis of stress-response strategies in Acidithiobacillus ferrooxidans ATCC 23270 exposed to organic extractant—Lix984n. World J Microbiol Biotechnol 28, 1045–1055 (2012). https://doi.org/10.1007/s11274-011-0903-3

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