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Effects of wetland degradation on bacterial community in the Zoige Wetland of Qinghai-Tibetan Plateau (China)

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Abstract

Wetland degradation makes significant impacts on soil, and bacterial communities in soil are likely to respond to these impacts. The purpose of this study was to investigate the impacts of soil property, soil type and soil depth on bacterial community in different stages of soil degradation in the Zoige Wetland. Microbial biomass carbon was estimated from chloroform fumigation-extraction. Bacterial communities were evaluated by cluster and principal component analysis of DGGE banding patterns and sequencing of partial 16S rDNA PCR amplicons. Experimental results showed that microbial biomass carbon decreased with the soil types (Peat soil > Swamp soil > Meadow soil > Sandy soil) and declined with soil depths (0–20 > 20–40 > 40–60 cm). Bacterial community was affected by soil type more primarily than by soil depth. In addition, the microbial biomass carbon was strongly correlated with soil water content, soil organic carbon and total nitrogen. Sequence analysis of DGGE bands indicated that bacterial phyla of α-Proteobacteria, γ-Proteobacteria, Bacteroidetes, Flavobacterium and Unidentified bacterium predominantly existed in the soil. All these results suggest that specific changes in soil property, soil type and soil depth affected soil bacterial community both quantitatively and qualitatively.

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Abbreviations

Pe:

Peat soil

Sw:

Swamp soil

Me:

Meadow soil

Sa:

Sandy soil

SWC:

Soil water content

SOC:

Soil organic carbon

TN:

Total nitrogen

TP:

Total phosphorus

TK:

Total kalium

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Acknowledgments

This project was supported by the 11th Five Years Key Programs for Science and Technology Development of China (No. 2007BAC18B03 and No. 2009BAI84B01).

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Authors and Affiliations

  1. Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, PR, China

    Jie Tang, Xiang Ding, Liming Wang, Qingrui Xu, Zhirong Yang, Jian Zhao, Qun Sun, Su Feng & Jie Zhang

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  1. Jie Tang
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  2. Xiang Ding
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  3. Liming Wang
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  4. Qingrui Xu
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  7. Qun Sun
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  8. Su Feng
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  9. Jie Zhang
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Correspondence to Jie Zhang.

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Jie Tang and Xiang Ding contributed equally to this research.

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Tang, J., Ding, X., Wang, L. et al. Effects of wetland degradation on bacterial community in the Zoige Wetland of Qinghai-Tibetan Plateau (China). World J Microbiol Biotechnol 28, 649–657 (2012). https://doi.org/10.1007/s11274-011-0858-4

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  • DOI: https://doi.org/10.1007/s11274-011-0858-4

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