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Technology for efficient and successful delivery of vermicompost colonized bioinoculants in Pogostemon cablin (patchouli) Benth.

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Abstract

The usefulness of vermicompost as a supporting media for growth of bioinoculants was evaluated for successful transfer of sufficient propagules of bioinoculants into the organic fields. The rooted plants after 50 days were pot and field tested for their growth and yield performances when transplanted along with rooting medium into pots/organic fields. The rooting medium, 50 days of inoculation, contained sufficient population of bioinoculants and arbuscular mycorrhizal (AM) fungi. Treatment with bioinoculants (except Trichoderma harzianum) substantially improved the root and shoot biomass of nursery raised rooted cuttings particularly in treatments containing Azotobacter chroococcum (150 and 91.67%, respectively), Glomus intraradices (117 and 91.67%, respectively) and Pseudomonas fluorescens (117 and 83%, respectively). The transplanted rooted plants in pots, over two harvests, yielded higher shoot biomass when rooting medium contained A. chroococcum (147%), G. intraradices (139%) and P. fluorescencs (139%). Although the treatments did not affect the content of essential oil, the quality of essential oil as measured by the content of patchouli alcohol improved with Glomus aggregatum (18%). Similar trends were observed in field trials with significantly higher biomass yield achieved with A. chroococcum (51%), G. intraradices (46%) and P. fluorescencs (17%) compared to control (un-inoculated) plots. Increased in herb yield was found to be related with increased nutrient uptake. The population of bioinoculants in the rhizosphere was observed to be considerably higher in plots receiving vermicompost enriched with bioinoculants. This technology can be a successful way of delivering sufficient propagules of bioinoculants along with vermicompost especially in organic fields.

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Acknowledgments

The authors are grateful to Director, CIMAP, Lucknow for facilities and encouragement, Dr. K. V. N. S. Srinivas (Phyto-chemist) for analyzing patchouli oil and to the Council of Scientific and Industrial Research (CSIR), New Delhi for providing funds.

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  1. Microbial Technology, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, Lucknow, 226015, India

    Rakshapal Singh, Ashutosh Awasthi & Alok Kalra

  2. Central Institute of Medicinal and Aromatic Plants (CSIR), Research Center, Allalasandra, Bangalore, 560065, India

    S. Divya

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  1. Rakshapal Singh
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Singh, R., Divya, S., Awasthi, A. et al. Technology for efficient and successful delivery of vermicompost colonized bioinoculants in Pogostemon cablin (patchouli) Benth.. World J Microbiol Biotechnol 28, 323–333 (2012). https://doi.org/10.1007/s11274-011-0823-2

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