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Effects of hydrogen peroxide on the motility, catalase and superoxide dismutase of dam and/or seqA mutant of Salmonella typhimurium

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Abstract

In addition to their role in the virulence attenuation of Salmonella and other pathogens, dam or seqA genes increase the sensitivity towards hydrogen peroxide. The aim of our study is to investigate the effect of H2O2 on the motility, the catalase and superoxide dismutase activities of dam and/or seqA mutants of Salmonella typhimurium. Our findings showed significant differences of the effects of H2O2 on the motility between wild type strain and all of mutants. Hydrogen peroxide changes SOD isoenzyme profile of these mutants by disappearance of Fe-SOD. Concerning the catalase, an increase of its activity was observed in the wild type, dam and seqA mutant. However, H2O2 decreases the activity of this enzyme in the double mutant strain. We can suggest that the dam gene, together with seqA, play a protective role in the oxidative stress response of Salmonella typhimurium.

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Acknowledgments

This work was supported by: « Ministère de l’Enseignement Supérieur; Faculté des Sciences de Bizerte-Département de Biologie » . We thank Pr. Casadesus J. for providing Salmonella strains.

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Authors and Affiliations

  1. Laboratoire de Traitement des Eaux Usées, Centre des Recherches et des Technologies des, Eaux (CERTE), Technopole Borj Cédria, BP 273, 8020, Soliman, Tunisia

    Abdelwaheb Chatti

  2. Département des Sciences de la vie, Faculté des Sciences de Bizerte, Unité de Biochimie des Lipides et interaction des macromolécules en Biologie, Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, 03/UR/0902, 7021, Zarzouna, Tunisia

    Abdelwaheb Chatti, Nadia Messaoudi, Mouadh Mihoub & Ahmed Landoulsi

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  1. Abdelwaheb Chatti
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  2. Nadia Messaoudi
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  3. Mouadh Mihoub
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  4. Ahmed Landoulsi
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Correspondence to Abdelwaheb Chatti.

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Chatti, A., Messaoudi, N., Mihoub, M. et al. Effects of hydrogen peroxide on the motility, catalase and superoxide dismutase of dam and/or seqA mutant of Salmonella typhimurium . World J Microbiol Biotechnol 28, 129–133 (2012). https://doi.org/10.1007/s11274-011-0801-8

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  • DOI: https://doi.org/10.1007/s11274-011-0801-8

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