Abstract
A new naringinase-producing strain, Jmudeb007 was indentified by means of morphological observation, gene homogeneous analysis and physiological and biochemical test. Its characteristics in expressing naringinase were investigated by batch culture in 7 L fermentors. Jmudeb007 grown white, circular, convex, and smooth edged colonies, and single, oval-shaped and nucleus contained cells. Its gene sequences of 26S rDNA D1/D2 region and 5.8S rDNA-ITS region both showed homogeneities at 99% to Williopsis californica. It appeared positive in glucose fermentation test, negative in urease test and diazo blue B (DBB) test. Strain Jmudeb007 was identified to W. californica. Cultivation of Jmudeb007 with three media containing different amount of glucose showed it was capable of expressing naringinase which hydrolyze naringin to naringenin. The naringinase synthesis of Jmudeb007 was regulated by glucose which depended on the concentration. Jumdeb007 could express naringinase in medium containing glucose no more than 4 g/L. The present work provides a new source of naringinase, W. californica Jmudeb007, which is non-pathogenic, convenient to conduct breeding operation, easy to develop fermentation process. It is the first report about yeast that can produce naringinase, which help to explore naringinase and other glycosidase from yeast.
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Thanks for foundation Supported by Key projects of Science and Technology Program of Fujian Province (2009N0044) and Chinese Scientific and technical services for enterprise projects (2009GJC40050) and foundation for innovative research team of Jimei University (2010A006).
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Hui Ni and Huinong Cai have made the equal contributions to the paper
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Ni, H., Li, L., Xiao, A. et al. Identification and characterization of a new naringinase-producing strain, Williopsis californica Jmudeb007. World J Microbiol Biotechnol 27, 2857–2862 (2011). https://doi.org/10.1007/s11274-011-0766-7
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DOI: https://doi.org/10.1007/s11274-011-0766-7