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Control of Fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici using leaf extract of Piper betle L.: a preliminary study

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Abstract

The main objective of this study was to evaluate the effectiveness of crude chloroform extract of Piper betle L. (PbC) in controlling Fusarium wilt of tomato (Lycopersicon esculentum) caused by Fusarium oxysporum f. sp. lycopersici. It was observed that 1% (w/w) amendment of the PbC in soil was more efficient in reducing the Fusarium population in soil than carbendazim and the combined amendment of carbendazim and PbC. Fusarium wilt control studies were carried out in a greenhouse. Variation in different parameters like shoot growth, root growth and mean fresh weights of tomato seedlings in all the treatments were recorded. Accumulation of total phenolics was also studied from the root tissues of tomato. Higher accumulation of total phenolics was observed in the Fusarium-infested plants as compared to that of healthy control and PbC-treated plants. Moreover, it was observed that the extract could reduce the symptoms and disease development. Electron microscopy studies were also done to observe the Fusarium infestation in the vascular bundles and to show the accumulation of total phenolics in the vacuoles of root tissue.

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Acknowledgments

We thank Dr. P. G. Rao, Director, NEIST, Jorhat and Dr. R. B. Srivastava, former Directorate of Life Sciences, DRDO, New Delhi for constant support and encouragement. We thank Dr. P. Chattopadhyay and Dr. R. S. Policegoudra for valuable guidance. Dr. B. G. Unni gratefully acknowledges Defence Research and Development Organization (DRDO), Govt. of India for financial support. I. M. Singha acknowledges DRDO for the SRF fellowship.

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Authors and Affiliations

  1. Biotechnology Division, Defence Research Laboratory (DRDO), Tezpur, 784 001, Assam, India

    Irom Manoj Singha, Jayshree Das, Ashok Naglot & Lokendra Singh

  2. Biotechnology Division, North-East Institute of Science and Technology (CSIR), Jorhat, 785 006, Assam, India

    Yelena Kakoty, Bala Gopalan Unni & Sawlang Borsingh Wann

  3. Department of Biotechnology, Gauhati University, Guwahati, 781 014, Assam, India

    Mohan Chandra Kalita

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  1. Irom Manoj Singha
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  2. Yelena Kakoty
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  3. Bala Gopalan Unni
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  4. Mohan Chandra Kalita
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  5. Jayshree Das
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  6. Ashok Naglot
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  7. Sawlang Borsingh Wann
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  8. Lokendra Singh
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Correspondence to Bala Gopalan Unni.

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Singha, I.M., Kakoty, Y., Unni, B.G. et al. Control of Fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici using leaf extract of Piper betle L.: a preliminary study. World J Microbiol Biotechnol 27, 2583–2589 (2011). https://doi.org/10.1007/s11274-011-0730-6

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  • DOI: https://doi.org/10.1007/s11274-011-0730-6

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