Abstract
A glucose utilizing strain, Arthrobacter A302 was used for cyclic adenosine monophosphate (cAMP) production in batch modes. The non-structured model in a 5 l stirred tank bioreactor for understanding, controlling, and optimizing the fermentation process was proposed using the logistic equation for microbial growth, the Luedeking-Piret equation for product formation and Luedeking-Piret-like equation for substrate uptake, respectively. The production of cAMP was a mixed-growth-associated pattern. Based on model prediction, a comparison of calculated value using the parameters evaluated above with another experimental data in 30 l bioreactor was used to test the model. The results predicted from the model were in good agreement with the experimental observations in 30 l bioreactor, which demonstrated that the model might be useful for the development and optimization of production of cAMP in industrial scale. Based on estimated kinetic parameters, three different fed-batch modes, constant rate and intermittent (once and repeated), were adopted in order to obtain more cAMP accumulation. Furthermore, the final production of cAMP reached 11.24 g l−1 after 72 h incubation using three stages feeding strategy. In particular, the cAMP productivity (0.156 g l−1 h−1) was successfully improved by 22.83, 11.43 and 9.86%, respectively, compared with the modes of the batch, constant rate fed-batch and intermittent fed-batch once.
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Abbreviations
- m :
-
Maintenance coefficient (g sugar per g biomass h−1)
- P :
-
Produced cAMP concentration (g l−1)
- P 0 :
-
Initial cAMP concentration (g l−1)
- S :
-
Glucose concentration (g l−1)
- S 0 :
-
Initial glucose concentration (g l−1)
- t :
-
Fermentation time (h)
- X :
-
Biomass concentration (g l−1)
- X m :
-
Maximum biomass concentration (g l−1)
- X 0 :
-
Initial biomass concentration (g l−1)
- Y P/S :
-
Yield coefficient of cAMP on glucose concentration (g per g sugar)
- Y X/S :
-
Yield coefficient of biomass on sugar (g per g sugar)
- α:
-
Growth-associated product formation coefficient (g per g biomass)
- β:
-
Non-growth-associated product formation coefficient (g per g biomass h−1)
- μ:
-
Specific growth rate (h−1)
- μ m :
-
Maximum specific growth rate (h−1)
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Acknowledgments
This work was supported by the Major Basic R&D Program of China (2007CB707803), the National High Technology Research and Development Program of China (2006AA020204), and China National Funds for Distinguished Young Scientists (21025625).
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Li, L., Chen, X., Ren, H. et al. Dynamic mathematical models of batch experiments and fed-batch cultures for cyclic adenosine monophosphate production by Arthrobacter A302. World J Microbiol Biotechnol 27, 2379–2385 (2011). https://doi.org/10.1007/s11274-011-0707-5
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DOI: https://doi.org/10.1007/s11274-011-0707-5