Skip to main content
SpringerLink
Log in

Storage conditions and animal source influence the dominant bacterial community composition in animal manure

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The aim of this study was to determine the impact of storage, animal diet, and animal source on the bacterial community composition of manure. The differences among bacterial community structures in fresh manure from cows on two different diets, cow manure stored in a deep pit for about one month, and fresh pig manure were compared. A molecular approach consisting of terminal restriction fragment length polymorphism (T-RFLP), in combination with sequence information from clone libraries, facilitated the identification of specific dominant bacterial populations that varied significantly among manures from different sources and treatments. One such population, represented by TRF 157, the most dominant peak of the bacterial community from stored manure, was identified as a Spirochaeta sp. Interestingly, this peak was absent in the fresh manure communities. The prevailing species in the fresh manure bacterial communities were distinct from those in manure from the storage pit, indicating a major shift in bacterial community composition induced by storage conditions. Moreover, distinct differences in bacterial communities were observed among animal source, but not animal feed. Manure storage is consequently an important parameter to consider when handling fertilizers, in order to obtain an optimal soil microbial ecosystem functioning.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Arthurson V (2009) Closing the global energy and nutrient cycles through application of biogas residue to agricultural land—potential benefits and drawback. Energies 2(2):226–242

    Article  CAS  Google Scholar 

  • Buret A, den Hollander N, Wallis PM, Befus D, Olson ME (1990) Zoonotic potential of Giardiasis in domestic ruminants. J Infect Dis 162:231–237

    Article  CAS  Google Scholar 

  • Burton CH, Turner C (2003) Manure management. Treatment strategies for sustainable agriculture, 2nd edn. Silsoe Research Institute, Bedford

    Google Scholar 

  • Canale-Parola E (1980) Revival of the names Spirochaeta litoralis, Spirochaeta zuelzerae, and Spirochaeta aurantia. Int J Syst Bact 30:594

    Article  Google Scholar 

  • Chapman PA, Siddons CA, Cerman Malo AT, Harkin MA (1997) A 1-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry. Epidemiol Infect 119:245–250

    Article  CAS  Google Scholar 

  • Cotta MA, Whitehead TR, Zeltwanger RL (2003) Isolation, characterization and comparison of bacteria from swine faeces and manure storage pits. Environ Microbiol 5(9):737–745

    Article  CAS  Google Scholar 

  • Davis RD, Carrington EG, Aitken MN, Fenlon DR, Svoboda I (1999) A user’s guide to research on application of organic wastes to land. Report number 11523-0, 1-129. Scotland and Northern Ireland Forum for Environmental Research, Marlow

  • Farzan A, Friendship RM, Cook A, Pollari F (2009) Occurrence of Salmonella, Campylobacter, Yersinia enterocolitica, Escherichia coli O157 and Listeria monocytogenes in swine. Zoonoses and public health

  • Hao X, Priya SM, Shah MA, Travis GR (2005) Influence of canola and sunflower diet amendments on cattle feedlot manure. J Environ Qual 34:1439–1445

    Article  CAS  Google Scholar 

  • Himathongkham S, Riemann H (1999) Destruction of Salmonella typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes in chicken manure by drying and/or gassing with ammonia. FEMS Microbiol Lett 171:179–182

    Article  CAS  Google Scholar 

  • Himathongkham S, Bahari S, Riemann H, Cliver DO (1999) Survival of Escherichia coli O157:H7 and Salmonella typhimurium in cow manure and cow manure slurry. FEMS Microbiol Lett 178:251–257

    Article  CAS  Google Scholar 

  • Ibekwe AM, Grieve CM, Lyon SR (2003) Characterization of microbial communities and composition in constructed dairy wetland wastewater effluent. Appl Environ Microbiol 69(9):5060–5069

    Article  CAS  Google Scholar 

  • Ingraham J, Maaloe L, Neidhardt FC (1983) Growth of the bacterial cell. Sinauer Associates Inc, Sunderland

    Google Scholar 

  • Jacobson LH, Nagle TA, Gregory NG, Bell RG, Roux GL, Haines JM (2002) Effect of feeding pasture-finished cattle different conserved forages on Escherichia coli in the rumen and faces. Meat Sci 62:93–106

    Article  Google Scholar 

  • Jones PW (1976) The effect of temperature, solids content and pH on the survival of Salmonellas in cattle slurry. British Vet J 132:284–293

    CAS  Google Scholar 

  • Jones K, Howard S, Wallace JS (1999) Intermittent shedding of thermophilic Campylobacters by sheep at pasture. J Appl Microbiol 86:531–536

    Article  CAS  Google Scholar 

  • Kearney TE, Larkin MJ, Levett PN (1993) The effect of slurry storage and anaerobic digestion on survival of pathogenic bacteria. J Appl Microbiol 74:86–93

    Article  CAS  Google Scholar 

  • Kowalchuk GA, Naoumenko ZS, Derikx PJL, Felske A, Stephen JR, Arkhipchenko IA (1999) Molecular analysis of ammonia-oxidizing bacteria of the B subdivision of the class Proteobacteria in compost and composted materials. Appl Environ Microbiol 65(2):396–403

    CAS  Google Scholar 

  • Kudva IT, Hunt CW, Williams CJ, Nance UM, Hovde CJ (1997) Evaluation of dietary influences on Escherichia coli O157:H7 shedding by sheep. Appl Environ Microbiol 63:3878–3886

    CAS  Google Scholar 

  • Kudva IT, Blanch K, Hovde CJ (1998) Analysis of Escherichia coli O157:H7 in ovine or bovine manure and manure slurry. Appl Environ Microbiol 64:3166–3174

    CAS  Google Scholar 

  • Leung K, Topp E (2001) Bacterial community dynamics in liquid swine manure during storage: molecular analysis using DGGE/PCR of 16S rDNA. FEMS Microbiol Ecol 38:169–177

    Article  CAS  Google Scholar 

  • Marti R, Dabert P, Pourcher AM (2009) Pig manure contamination marker selection based on the influence of biological treatment on the dominant fecal microbial groups. Appl Environ Microbiol 75(15):4967–4974

    Article  CAS  Google Scholar 

  • McDermid AS, McKee AS, Marsh PD (1988) Effect of environmental pH on enzyme activity and growth of Bacteroides gingivalis W50. Infect Immun 56(5):1096–1100

    CAS  Google Scholar 

  • McGee DA, Nielsen MK, Rasby RJ, Mader TL (2008) Effects of summer climatic conditions on body temperature in beef cows. Nebraska beef cattle reports, University of Nebraska, Lincoln

  • McGuirk SM (2002) Managing clostridial diseases in cattle. In: Proceedings of the 2002 midwest dairy herd health conference. University of Wisconsin School of Veterinary Medicine.

  • Morrison FB (1956) Feeds and feeding. The Morrison Publishing Co, Ithaca

    Google Scholar 

  • Muyzer G, Teske A, Wirsen CO, Jannasch HW (1995) Phylogenetic relationship of Thiomicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments. Arch Microbiol 164:162–172

    Article  Google Scholar 

  • Nicholson FA, Groves SJ, Chambers BJ (2003) Pathogen survival during livestock manure storage and following land application. Bioresour Technol 96:135–143

    Article  Google Scholar 

  • Odlare M, Pell M, Svensson K (2008) Changes in soil chemical and microbiological properties during 4 years of application of various organic residues. Waste Manag 28:1246–1253

    Article  CAS  Google Scholar 

  • Peu P, Brugere H, Pourcher AM, Kerouredan M, Godon JJ, Delgenes JP, Dabert P (2006) Dynamics of a pig slurry microbial community during anaerobic storage and management. Appl Environ Microbiol 72(5):3578–3585

    Article  CAS  Google Scholar 

  • Spoelstra SF (1978) Enumeration and isolation of anaerobic microbiota of piggery wastes. Appl Environ Microbiol 35:841–846

    CAS  Google Scholar 

  • Spoelstra SF (1980) Origin of objectionable odorous components in piggery wastes and the possibility of applying indicator components for studying odor development. Agric Environ 5:241–260

    Article  CAS  Google Scholar 

  • Spörndly R (1999) Fodertabeller för idisslare. Swedish Univeristy of Agricultural Sciences, Uppsala

    Google Scholar 

  • Stanley KN, Wallace JS, Jones K (1998) Thermophilic Campylobacters in dairy slurries on Lancashire farms: seasonal effects of storage and land application. J Appl Microbiol 85:405–409

    Article  Google Scholar 

  • Trochimchuk T, Fotheringham J, Topp E, Schraft H, Leung KT (2003) A comparison of DNA extraction and purification methods to detect Escherichia coli O157:H7 in cattle manure. J Microbiol Methods 54(2):165–175

    Article  CAS  Google Scholar 

  • Wang G, Zhao T, Doyle MP (1996) Fate of enterohemorrhagic Escherichia coli O157:H7 in bovine feces. Appl Environ Microbiol 62:2567–2570

    CAS  Google Scholar 

  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bact 173(2):697–703

    CAS  Google Scholar 

  • Whitehead TR, Cotta MA (2001) Characterisation and comparison of microbial populations in swine faeces and manure storage pits by 16S rDNA gene sequences analyses. Anaerobe 7:181–187

    Article  CAS  Google Scholar 

  • Yashurar A, Fuwa K, Jimbu M (1984) Identification of odorous compounds in fresh and rotten swine manure. Agric Biol Chem 48:3001–3010

    Article  Google Scholar 

  • Zhang RH, Day DL (1996) Anaerobic decomposition of swine manure and ammonia generation in a deep pit. Trans ASAE 39(5):1811–1815

    Google Scholar 

  • Zhao T, Doyle MP, Shere J, Garber L (1995) Prevalence of enterohemorrhagic Escherichia coli O157:H7 in a survey of dairy herds. Appl Environ Microbiol 61:1290–1293

    CAS  Google Scholar 

  • Zhu J (2000) A review of microbiology in swine manure odor control. Agric Ecosyst Environ 78:93–106

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS).

Author information

Authors and Affiliations

  1. Uppsala Biocenter, Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, 750 07, Uppsala, Sweden

    Veronica Arthurson

Authors
  1. Veronica Arthurson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Veronica Arthurson.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arthurson, V. Storage conditions and animal source influence the dominant bacterial community composition in animal manure. World J Microbiol Biotechnol 27, 2013–2022 (2011). https://doi.org/10.1007/s11274-011-0663-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-011-0663-0

Keywords

Search

Navigation