Abstract
Stachybotrys microspora is a filamentous fungus characterized by the secretion of multiple β-glucosidases. The production of these enzymes was studied under culture with variable carbon sources. The highest activity was produced on glucose (0.66 U ml−1) whereas galactose, lactose, cellobiose, Avicel cellulose, carboxymethylcellulose (CMC), wheat bran and gruel allowed intermediate production levels ranging between 0.08 and 0.48 U ml−1. The zymogram analysis showed that complex sugars such as Avicel cellulose and CMC induced the expression of several β-glucosidases whereas all tested simple sugars (mono and disaccharides) induced the expression of one single β-glucosidase, each time different. The most efficient β-glucosidase named bglG was produced on glucose which continues to be, at the same time, its strong inhibitor. The bglG N-terminal sequence confirmed that it is a novel β-glucosidase. According to its large molecular weight, this enzyme was assumed to belong to family 3 of β-glucosidases. RT-PCR analysis showed that family 3 expressions were induced on glucose while those of family 1 were repressed. Finally, with the use of different combinations of glucose and various carbon sources at different ratio, we showed that such sources direct the differential expression of β-glucosidases in S. microspora since our strain co-produced the β-glucosidases corresponding to each carbon source.
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Acknowledgments
This work is dedicated to the memory of Pr. Mokhtar Trifi. We thank Khmaies Benhaj Hafedh Belghith and Fatma Abdeljalil for their help in manuscript correction. Mosbeh Dardouri is thanked for his technical help. This work was supported by grants from the Ministry of Higher Education and Scientific Research, Tunisia.
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Saibi, W., Abdeljalil, S. & Gargouri, A. Carbon source directs the differential expression of β-glucosidases in Stachybotrys microspora . World J Microbiol Biotechnol 27, 1765–1774 (2011). https://doi.org/10.1007/s11274-010-0634-x
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DOI: https://doi.org/10.1007/s11274-010-0634-x