Abstract
The determination of the ideal cultivation conditions and the knowledge of solar radiation tolerance are important aspects that must be investigated for the use of Bipolaris euphorbiae Muchovej and Carvalho as a biocontrol agent. The present study was done to assess the growth rate, sporulation and viability of the fungus cultivated under different conditions of the initial pH value of the growth medium, temperature and photoperiod. The tolerance of this fungus’ conidia to light from a source simulating solar and ultraviolet radiation was also assessed. B. euphorbiae was affected by different pH values of the growth medium being 6.0 the adequate pH for fungus cultivation. Incubation of the fungus under temperatures of 22, 25 and 28°C enhanced mycelium growth, while sporulation and viability of conidia were better at 22 and 16°C. Different regimens of exposure to light of fungal cultures only affect sporulation. Conidia exposed to solar and ultraviolet radiation for 8 h and 90 min, respectively, presented viabilities higher than 92%, indicating high tolerance levels to radiation exposure.
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Acknowledgments
The authors would like to thank the National Council for Scientific and Technologic Development in Brazil (Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)), for financial support of the first author’s graduate studies. We are also grateful to Dr. José Carlos Barbosa and Dr. Newton La Scala Junior for assistance in the statistical analysis and solar and ultraviolet radiation experiments, respectively.
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Moraes, C., Monteiro, A.C. & Mochi, D.A. Reaction of Bipolaris euphorbiae to environmental factors and its tolerance to solar and ultraviolet radiation. World J Microbiol Biotechnol 27, 1449–1456 (2011). https://doi.org/10.1007/s11274-010-0597-y
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DOI: https://doi.org/10.1007/s11274-010-0597-y