Abstract
Aggregatibacter actinomycetemcomitans is a gram negative bacterium and an infectious agent of human diseases with severe oral and extra oral manifestations. One of the major virulence factors in this microorganism is cytolethal distending toxin (CDT). This toxin consists of three subunits—A, B, and C and is able to disrupt cell cycle by intrinsic DNAse activity of its B-subunit. Due to the fact that this protein can represent an important component of diagnostic, prophylactic and therapeutic preparations, production of CDT subunits in preparative quantities is of considerable practical importance. In the current study we demonstrated that deletion of NH2-terminal regions from the molecules of CDT-A, -B, or -C resulted in 20–400-fold increase in production of the corresponding subunits. These truncated molecules were used as immunogens to raise monospecific sera, which were shown in western blot to react specifically with the homologous subunits of cytolethal distending toxin.
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Acknowledgments
We wish to thank Dr. Annette Wittmer and Dr. Barbara Waidner, Department of Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany, for A. actinomycetemcomitans strains. Sequence determinations have been done in the “Genome Centre” IMB RAS, Moscow, Russia (http://www.genome-centre.narod.ru/).
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Vertieva, E., Kobozev, M., Tartakovskaya, D. et al. Genetic constructions, hyperexpressing recombinant fragments of cytolethal distending toxin of Aggregatibacter actinomycetemcomitans . World J Microbiol Biotechnol 27, 1189–1196 (2011). https://doi.org/10.1007/s11274-010-0567-4
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DOI: https://doi.org/10.1007/s11274-010-0567-4