Abstract
Leptospirosis is one of the diseases with economic impact, so its diagnosis and serosurveillance are very important for any control program. Many tests have been used in the field as screening tests of leptospirosis. The aim of this study was to evaluate the efficacy of combined recombinant antigens (rLipL41 + rLipL32) for serodiagnosis of canine leptospirosis and to compare the efficacy of IgG Enzyme linked immunosorbent assay and Latex agglutination test with standard MAT. A total of 533 canine serum samples were subjected to IgG-ELISA and LAT using recombinant LipL41 and LipL32 antigens in a single and in a combinations which were compared with standard MAT. The potential diagnostic cocktails of combined recombinant antigens (rLipL31 + rLipL41) developed in this study showed higher sensitivity and specificity in IgG ELISA and LAT (94.84, 88.50%; 98.21, 97.7%) when compared to the use of single recombinant antigen in this study. Results indicated that the both assays can be easily performed, and avoids the risk of infection in laboratory workers, and it seems to be a practical and suitable tool for serodiagnosis of leptospirosis.
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The authors acknowledge the funding agency, Department of Biotechnology, Govt. of India for its financial support to carry out this work.
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Subathra, M., Senthilkumar, T.M.A., Ramadass, P. et al. Evaluation of the cocktail recombinant antigens, LipL32 and LipL41 for serodiagnosis of canine leptospirosis. World J Microbiol Biotechnol 27, 1077–1082 (2011). https://doi.org/10.1007/s11274-010-0553-x
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DOI: https://doi.org/10.1007/s11274-010-0553-x