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Immunostimulatory and anti-tumor activity of a water-soluble polysaccharide from Phellinus baumii mycelia

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Abstract

A 17-kDa water-soluble polysaccharide (PB) was isolated and purified from Phellinus baumii using the DEAE-Sephadex A-50 and LPLC-Sephadex G-75 methods. Exposure of RAW264.7 macrophages to PB resulted in a significant increase of the cellular proliferation rate, nitric oxide production and expression levels of the IL-1β, IL-18, IL-6, IL-12p35 and IL-12p40 genes. An MTT assay indicated that PB markedly suppressed the proliferation of HepG2 human liver cancer cells in a dose-dependent manner. Cell cycle analysis demonstrated that PB caused cell cycle arrest at the S phase, and 400 μg/ml of PB induced apoptotic cell death in HepG2 cells after 48 h. The results suggested that PB inhibited the proliferation of HepG2 cells by inducing cell cycle arrest at S phase, leading to apoptosis. In summary, our data indicate that the PB exerts immunoregulatory and anti-tumor activities in vitro.

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Acknowledgments

This work was supported by the Chinese National Key Technology R&D Program (No. 2008BADA1B06) and the Chinese National Natural Science Foundation (No. 30970042, 30871767 and J0730647).

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Authors and Affiliations

  1. Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China

    Qin Xue, Jie Sun, Mingwen Zhao, Keyun Zhang & Ren Lai

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  1. Qin Xue
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  2. Jie Sun
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  3. Mingwen Zhao
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  4. Keyun Zhang
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  5. Ren Lai
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Correspondence to Mingwen Zhao.

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Xue, Q., Sun, J., Zhao, M. et al. Immunostimulatory and anti-tumor activity of a water-soluble polysaccharide from Phellinus baumii mycelia. World J Microbiol Biotechnol 27, 1017–1023 (2011). https://doi.org/10.1007/s11274-010-0545-x

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  • DOI: https://doi.org/10.1007/s11274-010-0545-x

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