Abstract
Blakeslea trispora produces carotenoids mixtures consisting mainly of lycopene, γ-carotene and β-carotene, together with trace amounts of other carotenoid precursors. The yield of these carotenoids and their composition are greatly affected by culture substrate. The scavenging capacity of carotenoids extract from cultures of B. trispora growing in various substrates was estimated using the 2,2-diphenyl-1-picrylhydrazyl method. Fractions enriched in β-carotene, γ-carotene and lycopene, obtained after column chromatography in alumina basic II, were also examined. Substrates containing starch and oils mixture, Ni2+, and that with pantothenic acid presented higher antioxidant activity. An increase in the antioxidant activity of the crude carotenoid extract compared to that of the isolated fractions enriched in β-carotene, γ-carotene and lycopene respectively, observed in most samples, indicated a possible synergistic effect. The results are of interest and by expanding this study to more substrates and other microorganisms- producing antioxidants, a formulation of extract with high free radical scavenging potential could be produced.
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References
Allen RG (1993) Free radicals and differentiation: the interrelationship of development and aging. In: Yu BP (ed) Free radicals in aging. CRC Press, London, pp 11–39
Ausich RL (1997) Commercial opportunities for carotenoid production by biotechnology. Pure Appl Chem 69:2169–2173
Britton G (1995) Chapter 2: UV/Visible Spectroscopy. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids Volume 1B: Spectroscopy. Birkhäuser Verlag, Basel, pp 13–62
Coleman HR, Chan CC, Ferris FL III, Chew EY (2008) Age-related macular degeneration. Lancet 372:1835–1845
Craft NE (1992) Carotenoid reversed-phase high-performance liquid chromatography methods: reference compendium. Meth Enzymol 213:185–201
Di Mascio P, Kaiser S, Sies H (1989) Lycopene as most efficient biological carotenoid singlet oxygen quencher. Arch Biochem Biophys 274:532–538
Echaverri-Erasum C, Johnson EA (2002) Fungal carotenoids. In: Khachatourians GG, Arora DK (eds) Applied mycology and biotechnology. Elsevier, Amsterdam, pp 45–87
El-Agamey GM, Lowe A, McGarvey DJ, Mortensen A, Phillip DM, Truscott TG, Young AJ (2004) Carotenoid radical chemistry and antioxidant/pro-oxidant properties. Arch Biochem Biophys 430:37–48
Erdman JW, Ford NA, Lindshield BL (2009) Are the health attributes of lycopene related to its antioxidant function? Arch Biochem Biophys 483:229–235
Fang YZ, Yang S, Wu G (2002) Free radicals, antioxidants, and nutrition. Nutrition 18:872–879
Feofilova EP, Tereshina VM, Memorskaya AS, Dul’kin LM, Goncharov NG (2006) Fungal lycopene: the biotechnology of its production and prospects for its application in medicine. Microbiology 75:629–633
Fuhrman B, Ben-Yaish L, Attias J, Hayek T, Aviram M (1997) Tomato lycopene and β-carotene inhibit low density lipoprotein oxidation and this effect depends on the lipoprotein vitamin E content. Nutr Metabol Cardiovasc Dis 7:433–443
Fuhrman B, Volkova N, Rosenblat M, Aviram M (2000) Lycopene synergistically inhibits LDL oxidation in combination with vitamin E, glabridin, rosmarinic acid, carnosic acid, or garlic. Antioxid Red Signal 2:491–505
Halliwell B (1997) Antioxidants and human disease: a general introduction. Nutr Rev 5:544–552
Halliwell B, Gutteridge JMC (1990) Role off free radicals and catalytic metal ions in human diseases. Meth Enzymol 186:1–85
Halliwell B, Murcia MA, Chirico S, Aruoma OI (1995) Free radicals and antioxidants in food and in vivo: what they do and how they work. Crit Rev Food Sci Nutr 35:7–20
Hirschberg J (1999) Production of high-value compounds: carotenoids and vitamin E. Cur Opin Biotech 10:186–191
Jacob RA (1995) The integrated antioxidant system. Nutr Res 15:755–766
Kiokias S, Gordon MH (2004) Antioxidant properties of carotenoids in vitro and in vivo. Food Rev Inter 20:99–121
Kohlmeier L, Hasting SB (1995) Epidemiologic evidence of a role of carotenoids in cardiovascular disease prevention. Am J Clin Nutr 62:137S–143S
Lee JH, Ozcelik B, Min DB (2003) Electron donation mechanisms of β-carotene as a free radical scavenger. J Food Sci 68:861–865
Lian F, Wang XD (2008) Enzymatic metabolites of lycopene induce Nrf2-mediated expression of phase II detoxifying/antioxidant enzymes in human bronchial epithelial cells. Inter J Canc 123:1262–1268
Liu D, Shi J, Ibarra AC, Kakuda Y, Xue SJ (2008) The scavenging capacity and synergistic effects of lycopene, vitamin E, vitamin C, and β-carotene mixtures on the DPPH free radical. LWT 41:1344–1349
Lopez-Nieto M, Costa J, Peiro E, Méndez E, Rodriguez-Sálz M, de la Fuente JL, Cabri W, Barredo JL (2004) Biotechnological lycopene production by mated fermentation of Blakeslea trispora. Appl Microbiol Biotechnol 66:153–159
Mantzouridou F, Tsimidou MZ (2007) Carotenoid pattern in Blakeslea trispora grown on oil-enriched substrates with regard to triacylglycerol species accumulation. Eur J Lip Sci Technol 109:3–10
Mares-Perlman JA, Brady WE, Klein R, Klein BEK, Bowen P, Stacewicz-Sapuntzakis M, Palta M (1995) Serum antioxidants and age-related macular degeneration in a population-based case-control study. Arch Ophthalmol 113:1518–1523
Matos HR, Capelozzi VL, Gomes OF, Di Mascio P, Medeiros MHG (2001) Lycopene inhibits DNA damage and liver necrosis in rats treated with ferric nitrilotriacetate. Arch Biochem Biophys 396:171–177
Mein JR, Lian F, Wang XD (2008) Biological activity of lycopene metabolites: implications for cancer prevention. Nutr Rev 66:667–683
Mensor LL, Menezes FS, Leitao GG, Reis AS, dos Santos TC, Coube CS et al (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 15:127–130
Molyneux P (2004) The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanak J Sci Technol 26:211–219
Namiki M (1990) Antioxidants/antimutagens in food. Crit Rev Food Sci Nutr 29:273–300
Nishino H, Murakoshi M, Tokuda H, Satomi Y (2009) Cancer prevention by carotenoids. Arch Biochem Biophys 483:165–168
Olson JA, Krinsky NI (1995) Introduction: the colorful, fascinating world of the carotenoids: important physiologic modulators. FASEB J 9:1547–1550
Papaioannou EH, Liakopoulou-Kyriakides M (2010) Substrate contribution on carotenoids production in Blakeslea trispora cultivations. Food Bioprod Process 88:305–311
Papaioannou E, Roukas T, Liakopoulou-Kyriakides M (2008) Effect of biomass pre-treatment and solvent on β-carotene and lycopene selective extraction from Blakeslea trispora cells. Prep Biochem Biotechnol 38:246–256
Papaioannou EH, Liakopoulou-Kyriakides M, Christofilos D, Arvanitidis I, Kourouklis G (2009) Raman spectroscopy for intracellular monitoring of carotenoid in Blakeslea trispora. Appl Biochem Biotechnol 159:478–487
Rodriguez-Amaya DB (2001) A guide to carotenoid analysis in foods. OMNI Research. ILSI Press, Washington, DC, pp 14–22
Rodriquez-Amaya DB (2003) Carotenoids. In: Caballero B (ed) Encyclopedia of food science and nutrition. Academic Press, New York, pp 927–943
Seddon JM, Ajani UA, Sperduto RD, Hiller R, Blair N, Burton TC, Farber MD, Gragoudas ES, Haller J, Miller DT (1994) Dietary carotenoids, vitamins A, C, and E, and advanced age-related macular degeneration. Eye disease case-control study group. J Am Med Assoc (JAMA) 272:1413–1420
Shixian Q, Dai Y, Kakuda Y, Shi J, Mittal G, Yeung D, Jiang Y (2005) Synergistic anti-oxidative effects of lycopene with other bioactive compounds. Food Rev Inter 21:295–311
Sies H (2007) Total antioxidant capacity: appraisal of a concept. J Nutr 137:1493–1495
Stahl W, Junghans A, de Boer DE, Briviba K, Sies H (1998) Carotenoid mixtures protect multilamellar liposomes against oxidative damage: synergistic effects of lycopene and lutein. FEBS Lett 427:305–308
Takaichi S, Ishidsu JI (1992) Characterization of carotenoids in photosynthetic bacteria. Meth Enzymol 213:374–386
Tereshina VM, Memorskaya AS, Feovilova EP (2002) Dormant cells in the development cycle of Blakeslea trispora: distinct patterns of the lipid and carbohydrate composition. Microbiol 71:684–689
Tereshina VM, Memorskaya AS, Feovilova EP (2003) Zygote formation in Blakeslea trispora: morphological peculiarities and relationship with carotenoid synthesis. Microbiology 72:448–454
Tereshina VM, Memorskaya AS, Feofilova EP (2010) Lipid composition of Zygomycetous fungi Blakeslea trispora under stimulation of lycopene synthesis. Microbiology 79:34–39
Vandamme JE (1992) Production of vitamins, coenzymes and related biochemicals by biotechnological processes. Chem Technol Biotechnol 53:313–327
Varzakakou M, Roukas T, Kotzekidou P (2010) Effect of the ratio of (+) and (−) mating type of Blakeslea trispora on carotene production from cheese whey in submerged fermentation. World J Microbiol Biotechnol. ISSN 0959-3993(Print) 1573-0972
Verlangieri AJ, Kapeghian JC, El-Dean S, Bush M (1985) Fruit and vegetable consumption and cardiovascular mortality. Med Hypoth 16:7–15
Weisburger JH (2002) Lycopene and tomato products in health promotion. Exp Biol Med 227:924–927
Werkman TA, Vam den Ende H (1973) Trisporic acid synthesis in Blakeslea trispora interaction between plus and minus mating types. Arch Microbiol 90:365–374
Wertz K, Siler U, Goralczyk R (2004) Lycopene: modes of action to promote prostate health. Arch Biochem Biophys 430:127–134
Young AJ, Lowe GM (2001) Antioxidants and prooxidants properties of carotenoids. Arch Biochem Biophys 385:20–27
Young AJ, Phillip DM, Lowe GM (2004) Carotenoid antioxidant activity. In: Krinsky NI, Mayne ST, Sies H (eds) Carotenoids in health and disease. Marcel Dekkar, New York
Ziegler RG, Taylor-Mayne S, Swanson CA (1996) Nutrition and lung cancer. Canc Caus Cont 7:157–177
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Papaioannou, E.H., Stoforos, N.G. & Liakopoulou-Kyriakides, M. Substrate contribution on free radical scavenging capacity of carotenoid extracts produced from Blakeslea trispora cultures. World J Microbiol Biotechnol 27, 851–858 (2011). https://doi.org/10.1007/s11274-010-0527-z
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DOI: https://doi.org/10.1007/s11274-010-0527-z