Isolation, characterization, and bioactivity of endophytic fungi of Tylophora indica

  • Susheel Kumar
  • Nutan Kaushik
  • Ruangelie Edrada-Ebel
  • Rainer Ebel
  • Peter Proksch
Original Paper


Dothediomycetes sp., Alternariatenuissima, Thielaviasubthermophila, Alternaria sp., Nigrosporaoryzae, Colletotrichum truncatum, and Chaetomium sp. were isolated as endophytic fungi from leaves and stems of the medicinal plant, Tylophora indica, based on rDNA sequencing of ITS region and microscopic examination. Alternariatenuissima, Colletotrichum truncatum, and Alternaria sp. were found to be active against both Sclerotiniasclerotiorum and Fusariumoxysporum. Chaetomium sp. showed very mild activity against Sclerotiniasclerotiorum and Fusariumoxysporum. Whereas Dothideomycete sp. and Thielaviasubthermophila showed high activity against Sclerotiniasclerotiorum. Methanol extract of Dothediomycetes sp. showed 66.5% growth inhibition (GI) at 500 μg/ml.


Endophytic fungi Tylophora indica Sclerotinina Bioassay 



Authors are thankful to DST/DAAD for providing partial financial support for exchange visits. Thanks are due to late Prof. K G Mukherjee for identification of fungi. Susheel Kumar is grateful to University Grant Commission, New Delhi, India, for providing Research Fellowship.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Susheel Kumar
    • 1
  • Nutan Kaushik
    • 2
  • Ruangelie Edrada-Ebel
    • 3
  • Rainer Ebel
    • 3
    • 4
  • Peter Proksch
    • 3
  1. 1.TERI UniversityVasant Kunj, New DelhiIndia
  2. 2.The Energy and Resources Institute (TERI), India Habitat CentreNew DelhiIndia
  3. 3.Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine-UniversitätDüsseldorfGermany
  4. 4.Department of ChemistryUniversity of AberdeenScotlandUK

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