Abstract
Monascus-fermented products have been widely used in Taiwan and other Asian countries as health foods. Unfortunately, many Monascus strains concurrently produce trace amounts of toxic citrinin. This study isolated a strain NPUST-B11 with the ability to degrade citrinin as the only carbon source. The isolated strain NPUST-B11 was characterised and identified as Klebsiella pneumoniae by 16S rRNA gene analysis using UNI-F and UNI-R primers. The isolated strain was then incubated in the mineral broth containing 10 ppm of citrinin, 1.2% of glucose, 0.3% of peptone and 100 ppm of vitamin C under optimal conditions, including pH 7, 200 rpm and 37°C. Citrinin was rapidly degraded with incubation from 97.9% at 1 h to 8.67% at 5 h and completely depleted at 10 h. Overall, this strain could be useful for the degradation of citrinin in food products and other medical applications.
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Acknowledgments
We thank Emeritus Professor Der-Feng Teng, the Department of Food Science, NPUST for his assistance with data collection.
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Chen, YH., Sheu, SC., Mau, JL. et al. Isolation and characterization of a strain of Klebsiella pneumoniae with citrinin-degrading activity. World J Microbiol Biotechnol 27, 487–493 (2011). https://doi.org/10.1007/s11274-010-0478-4
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DOI: https://doi.org/10.1007/s11274-010-0478-4