Biodesulfurization activity can be enhanced by assembling nano-γ-Al2O3 particles on the magnetic immobilized Rhodococcus erythropolis LSSE8-1-vgb. The cells can be collected and reused conveniently by an external magnetic field. Firstly, cells were magnetic immobilized by coating with Fe3O4 nano-particles. The optimal ratio of cells to magnetic Fe3O4 nano-particles was determined to be 50:1 (g/g). Then nano-γ-Al2O3 adsorbents were assembled onto the cells to enhance the desulfurization activity. The nano-γ-Al2O3 adsorbent had the largest pore volume as well as specific surface area, and the strongest electrostatics interaction with microbial cell, and cells assembled with this nano-adsorbent performed the highest desulfurization activity. The activity of magnetic immobilized cells assembled with adsorbents was tested in desulfurization of model oil. The desulfurization rate was raised by nearly 20% when the amount ratio of magnetic particles to adsorbents was 1:5 (g/g). These cells can be reused. The activity decreased less than 10% through out three desulfurization-activation-reuse recycles.
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The authors would like to acknowledge the financial supports by the State Major Basic Research Development Program of China (Grant No. 2006CB202507) and the National Natural Science Foundation of China (No. 30970046).
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Zhang, T., Li, WL., Chen, XX. et al. Enhanced biodesulfurization by magnetic immobilized Rhodococcus erythropolis LSSE8-1-vgb assembled with nano-γ-Al2O3 . World J Microbiol Biotechnol 27, 299–305 (2011). https://doi.org/10.1007/s11274-010-0459-7
- Magnetic immobilization
- In-situ integrated desulfurization