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Potential plant growth-promoting activity of Serratia nematodiphila NII-0928 on black pepper (Piper nigrum L.)

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Abstract

A potential bacterial strain designated as NII-0928 isolated from Western ghat forest soil with multiple plant growth promoting attributes, and it has been identified and characterized. Plant growth promoting traits were analyzed by determining the P-solubilization efficiency, Indole acetic acid production, HCN, siderophore production and growth in nitrogen free medium. It was able to solubilize phosphate (76.6 μg ml−1), and produce indole acetic acid (58.9 μg ml−1) at 28 ± 2°C. Qualitative detection of siderophore production and HCN were also observed. At 5°C it was found to express all the plant growth promotion attributes except HCN production. The ability to colonize roots is a sine qua non condition for a rhizobacteria to be considered a true plant growth-promoting rhizobacteria (PGPR). 16S rRNA gene sequencing reveals the identity of the isolate as Serratia nematodiphila with which it shares highest sequence similarity (99.4%). Seed bacterization with black pepper cuttings in greenhouse trials using Sand: Soil: FYM with three individual experimental sets with their respective control showed clearly the growth promoting activity. Hence, Serratia nematodiphila NII-0928 is a promising plant growth promoting isolate showing multiple PGPR attributes that can significantly influence black pepper cuttings. The result of this study provides a strong basis for further development of this strain as a bioinoculants to attain the desired plant growth promoting activity in black pepper growing fields.

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Acknowledgments

The authors would like to thank CSIR Task force network programme on Exploration of India’s Rich Microbial Diversity (NWP 0006) for providing the financial support.

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Authors and Affiliations

  1. Biotechnology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Industrial Estate, Pappanamcode, Trivandrum, 695019, India

    Syed G. Dastager, C. K. Deepa & Ashok Pandey

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  1. Syed G. Dastager
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  2. C. K. Deepa
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  3. Ashok Pandey
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Correspondence to Syed G. Dastager or Ashok Pandey.

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Syed G. Dastager and C. K. Deepa contributed equally to the work.

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Dastager, S.G., Deepa, C.K. & Pandey, A. Potential plant growth-promoting activity of Serratia nematodiphila NII-0928 on black pepper (Piper nigrum L.). World J Microbiol Biotechnol 27, 259–265 (2011). https://doi.org/10.1007/s11274-010-0454-z

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