The Klebsiella sp. strain ATCC13883T capable of degrading carbofuran phenol (2,3-dihydro-2,2-dimethylbenzofuran-7-ol) has been separated from the soil by enrichment culture technique and immobilized in various, namely polyurethane foam (PUF), polyacrylamide, alginate, agar and alginate-bentonite clay-powdered activated charcoal (PAC). The degradation rates of 20 and 30 mM carbofuran phenol by free and immobilized cells in batch and semi-continuous shaken cultures were compared. The PUF-immobilized cells achieved higher degradation rates in a shorter time than freely suspended cells and the cells immobilized in polyacrylamide, alginate and agar. The PUF- and alginate-bentonite clay-PAC-immobilized cells could be reused for more than 36 cycles, polyacrylamide-entrapped cells for 20 cycles and alginate-bentonite-PAC 28 cycles, without losing any degradation capacity and showed better tolerance to pH, temperature and concentration changes than free cells. These results showed that cells immobilized in modified alginate-bentonite-PAC immobilizers tolerated and completely degraded carbofuran phenol at initial concentrations of 20 and 30 mM and also higher. Such a bacterial strain could be used for bioremediation of environments contaminated with phenolic compounds.
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The authors wish to express all their gratitude to University Sophisticated Instrumentation Centre (USIC), Karnatak University, Dharwad for providing instrumental facilities.
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Kadakol, J.C., Kamanavalli, C.M. & Shouche, Y. Biodegradation of Carbofuran phenol by free and immobilized cells of Klebsiella pneumoniae ATCC13883T. World J Microbiol Biotechnol 27, 25–29 (2011). https://doi.org/10.1007/s11274-010-0422-7
- Carbofuran phenol
- Klebsiella sp. strain ATCC13883T
- Immobilized cells
- Polyurethane foam