World Journal of Microbiology and Biotechnology

, Volume 26, Issue 11, pp 2089–2092 | Cite as

In vitro activity of water-soluble quaternary chitosan chloride salt against E. coli

  • Leandro Prezotto da Silva
  • Douglas de Britto
  • Mirna Helena Regali Seleghim
  • Odilio B. G. Assis
Short Communication


The antibacterial effect of the N,N,N-trimethyl chitosan chloride salt (TMC) was evaluated against the bacterium Escherichia coli. The derivative was prepared via reaction of chitosan with dimethyl sulfate in the presence of NaOH and the Minimum Inhibitory Concentration was assessed by measuring the changes in turbidity and by counting of Colony Forming Units (c.f.u.). The results indicated good antibacterial activity against E. coli for all concentration of TMC tested (20.0; 7.5 and 3.5 mg/l) over 7 h of incubation. From the data, the ideal lethal concentration was determined as 20.0 mg/l.


Escherichia coli Chitosan Water-soluble chitosan Minimum inhibitory concentration 


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Leandro Prezotto da Silva
    • 1
    • 2
  • Douglas de Britto
    • 1
  • Mirna Helena Regali Seleghim
    • 2
  • Odilio B. G. Assis
    • 1
  1. 1.Embrapa Instrumentação AgropecuáriaSão CarlosBrazil
  2. 2.Universidade Federal de São CarlosSão CarlosBrazil

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