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In vitro activity of water-soluble quaternary chitosan chloride salt against E. coli

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Abstract

The antibacterial effect of the N,N,N-trimethyl chitosan chloride salt (TMC) was evaluated against the bacterium Escherichia coli. The derivative was prepared via reaction of chitosan with dimethyl sulfate in the presence of NaOH and the Minimum Inhibitory Concentration was assessed by measuring the changes in turbidity and by counting of Colony Forming Units (c.f.u.). The results indicated good antibacterial activity against E. coli for all concentration of TMC tested (20.0; 7.5 and 3.5 mg/l) over 7 h of incubation. From the data, the ideal lethal concentration was determined as 20.0 mg/l.

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Acknowledgments

The authors gratefully acknowledge the support of Embrapa (Rede AgroNano), FAPESP and CNPq (Brazilian Science Agencies).

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Authors and Affiliations

  1. Embrapa Instrumentação Agropecuária, Rua XV de Novembro 1452, 13560-970, São Carlos, SP, Brazil

    Leandro Prezotto da Silva, Douglas de Britto & Odilio B. G. Assis

  2. Universidade Federal de São Carlos, UFSCar, São Carlos, SP, Brazil

    Leandro Prezotto da Silva & Mirna Helena Regali Seleghim

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  1. Leandro Prezotto da Silva
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  2. Douglas de Britto
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  3. Mirna Helena Regali Seleghim
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  4. Odilio B. G. Assis
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Correspondence to Odilio B. G. Assis.

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Silva, L.P.d., de Britto, D., Seleghim, M.H.R. et al. In vitro activity of water-soluble quaternary chitosan chloride salt against E. coli . World J Microbiol Biotechnol 26, 2089–2092 (2010). https://doi.org/10.1007/s11274-010-0378-7

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  • DOI: https://doi.org/10.1007/s11274-010-0378-7

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