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A unique thermostable and organic solvent tolerant lipase from newly isolated Aneurinibacillus thermoaerophilus strain HZ: physical factor studies

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Abstract

A newly isolated thermophilic bacterium, Aneurinibacillus thermoaerophilus strain HZ, from a hot spring recreational area (Sungai Kelah, Malaysia), showed an extracellular lipase activity. It was identified based on 16S rRNA sequencing, where phylogenetic analysis revealed its homology to Aneurinibacillus thermoaerophilus. The strain produced a lipase that was stable in various organic solvents such as dimethyl sulfoxide, toluene, p-xylene, and hexane. In order to increase lipase production, optimization of physical factors which affected the growth and lipase production was studied. The optimal growth was obtained at 50°C and pH 8.0; while the maximal lipase production was achieved in the logarithmic decline phase at 60°C and pH 7.5 with 7% starting inoculum and 150 rev/min shaking rate for 48 h incubation.

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Acknowledgment

This work was supported by the Sciencefund Grant No. 02-01-04-SF0212 from the Malaysian Ministry of Science/Technology and Innovation (MOSTI).

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Authors and Affiliations

  1. Enzyme and Microbial Technology Research, Department of Microbiology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Malihe Masomian & Raja Noor Zaliha Raja Abd Rahman

  2. Enzyme and Microbial Technology Research, Department of Biochemistry, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Abu Bakar Salleh

  3. Enzyme and Microbial Technology Research, Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Mahiran Basri

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  1. Malihe Masomian
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  2. Raja Noor Zaliha Raja Abd Rahman
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  3. Abu Bakar Salleh
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  4. Mahiran Basri
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Correspondence to Raja Noor Zaliha Raja Abd Rahman.

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Masomian, M., Rahman, R.N.Z.R.A., Salleh, A.B. et al. A unique thermostable and organic solvent tolerant lipase from newly isolated Aneurinibacillus thermoaerophilus strain HZ: physical factor studies. World J Microbiol Biotechnol 26, 1693–1701 (2010). https://doi.org/10.1007/s11274-010-0347-1

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