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Physiological and molecular biological characteristics of heterotrophic ammonia oxidation by Bacillus sp. LY

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Abstract

This work concerned the study of ammonia oxidation by a heterotrophic bacterium, Bacillus sp. LY, isolated from a membrane bioreactor (MBR) treating a synthetic domestic wastewater with 15-l working volume. During the batch test 74.8% of ammonium and 61.0% of CODCr were removed, and the maximum nitrification rate was 173.6 mgN/(g dry weight day). The ammonia oxidation ability was inhibited by high organic substrate concentrations and was highest in the poorest medium. The isolate oxidized ammonia to NH2OH, and the presence of ammonia monooxygenase (AMO) in Bacillus sp. LY was further confirmed by a specific 491-bp fragment of the amoA gene, but the results of PCR amplification suggest that amoB may not be a member of the amo operon in this isolate. Moreover, from the nitrogen balance, the percentage of nitrogen lost in a batch test was estimated to be 61.9%, which was presumed to have been removed via aerobic denitrification.

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Yan Lin, Hainan Kong, Deyi Wu & Chunjie Li

  2. Asian Center for Environmental Research (ACER), Meisei University, Tokyo, 191-8506, Japan

    Yan Lin & Shuzo Tanaka

  3. Shanghai Apeloa Pharmaceutical Research Institute, Shanghai, 201314, People’s Republic of China

    Rongying Wang

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  1. Yan Lin
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  2. Hainan Kong
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  3. Deyi Wu
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  4. Chunjie Li
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  6. Shuzo Tanaka
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Correspondence to Yan Lin.

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Lin, Y., Kong, H., Wu, D. et al. Physiological and molecular biological characteristics of heterotrophic ammonia oxidation by Bacillus sp. LY. World J Microbiol Biotechnol 26, 1605–1612 (2010). https://doi.org/10.1007/s11274-010-0336-4

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  • DOI: https://doi.org/10.1007/s11274-010-0336-4

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