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Investigation on the potential application of biological agents in the extension of shelf life of fresh beef in Nigeria

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Abstract

The objective of this study was to evaluate the ability of lactic acid bacteria (LAB) cultures to preserve fresh beef at room temperature, with a view to promoting safety and availability of the product in Nigeria. Two LAB strains, Pediococcus pentosaceus LIV 01 and P. acidilactici FLE 01, were applied as starters (106 cfu/g) on sliced fresh beef samples, and were stored for 7 days at 30°C. Analyses of microbiological, thiobarbituric acid (TBA) and free fatty acids (FFA) were carried out during storage. Results indicated reduction in the Enterobacteriaceae, Staphylococcus and coliforms in starter inoculated samples. TBA and FFA were lower in starter culture inoculated samples compared to controls during storage. In a challenge experiment against the LAB cultures during a 7-day storage, two sets of meat were inoculated separately with 106 cfu/g each of pathogenic organisms Listeria monocytogenes and Salmonella Typhimurium. There was about 1 log reduction in the L. monocytogenes on day 1 while counts were below detection limit (<2 log) on day 2 in meat samples inoculated with P. pentosaceus alone and in combination with P. acidilactici. Counts of S. Typhimurium showed about 2 log reduction in starter inoculated samples during storage while an increase by about 3 log was observed in control samples. The protective ability of the LAB strains could be exploited in shelf life extension and control of foodborne pathogens in fresh beef; their use as biological preservatives may help in promoting public health, safety and availability of the product in Nigeria.

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Acknowledgments

The authors are grateful to the School of Biosciences, University of Nottingham, UK, where part of this research study was carried out.

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Authors and Affiliations

  1. Food Technology Department, The Federal Polytechnic, PMB 420, Offa, Kwara State, Nigeria

    Olusegun A. Olaoye

  2. Microbial Physiology and Biochemistry Unit, Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria

    Abiodun A. Onilude

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  1. Olusegun A. Olaoye
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  2. Abiodun A. Onilude
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Correspondence to Olusegun A. Olaoye.

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Olaoye, O.A., Onilude, A.A. Investigation on the potential application of biological agents in the extension of shelf life of fresh beef in Nigeria. World J Microbiol Biotechnol 26, 1445–1454 (2010). https://doi.org/10.1007/s11274-010-0319-5

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  • DOI: https://doi.org/10.1007/s11274-010-0319-5

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