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Influence of ultrasound amplitude and duty cycle on fungal morphology and broth rheology of Aspergillus terreus

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Abstract

Effects of ultrasound amplitude and duty cycle on cultures of Aspergillus terreus are reported in a 25 l slurry bubble column sonobioreactor. Fermentations were carried out batchwise. A 2k-factorial design with added central points was used. Sonication at any cycle and amplitude level did not affect biomass growth rate and yield relative to nonsonicated control, but did affect growth morphology. Ultrasound disrupted fungal pellets and caused the biomass to grow mainly as dispersed hyphae. Production of lovastatin was reduced by medium- and high-cycle sonication. Sonication affected broth rheology. In view of these results, sonication can be used to modify growth morphology and broth rheology without affecting growth rate and yield of filamentous fungi.

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Acknowledgments

This research was supported by the Ministerio de Educación y Ciencia (Grant CTQ2004-04454/PPQ) and Junta de Andalucía (Grant BIO 263), Spain.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Almería, 04120, Almería, Spain

    N. Sainz Herrán, J. L. Casas López & J. A. Sánchez Pérez

  2. School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand

    Y. Chisti

Authors
  1. N. Sainz Herrán
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  2. J. L. Casas López
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  3. J. A. Sánchez Pérez
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  4. Y. Chisti
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Correspondence to J. L. Casas López.

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Sainz Herrán, N., Casas López, J.L., Sánchez Pérez, J.A. et al. Influence of ultrasound amplitude and duty cycle on fungal morphology and broth rheology of Aspergillus terreus . World J Microbiol Biotechnol 26, 1409–1418 (2010). https://doi.org/10.1007/s11274-010-0315-9

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  • DOI: https://doi.org/10.1007/s11274-010-0315-9

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