Abstract
Sustained oscillations have been observed in cell growth and glycerol metabolism by Klebsiella pneumoniae during long-term continuous cultivations under anaerobic conditions. However, the oscillation behavior of glucose–glycerol co-fermentation under micro-aerobic conditions has yet not been studied. In this study, the unclear mechanism of oscillation was investigated by using different substrates including glycerol, glucose, and mixtures of glycerol and glucose. A large perturbation of substrate concentration was operated to study the dynamic behavior of continuous fermentation by K. pneumoniae DSM 2026 under micro-aerobic conditions. Oscillations occurred when 80 g of glycerol l−1 was used as single carbon source or co-substrate with 35 g of glucose l−1, whereas no oscillation was observed when glucose was used alone. During the oscillation phases, parameters periodically passed maxima and minima at a relatively constant time interval, and the period of oscillation was 10–16 h. The comparison between glycerol and glucose metabolism revealed that a potential mechanism of oscillation lies in the accumulation of 3-hydroxypropionaldehyde, which is toxic to cell growth.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. 20576018) and the grant from the Major State Basic Research Development Program of China (973 Program) (No. 2007CB714306).
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Sun, LH., Song, ZY., Sun, YQ. et al. Dynamic behavior of glycerol–glucose co-fermentation for 1,3-propanediol production by Klebsiella pneumoniae DSM 2026 under micro-aerobic conditions. World J Microbiol Biotechnol 26, 1401–1407 (2010). https://doi.org/10.1007/s11274-010-0314-x
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DOI: https://doi.org/10.1007/s11274-010-0314-x