Abstract
An AHL lactonase gene (aiiA) was PCR amplified from the genomic DNA of Bacillus amyloliquefaciens, with the intact open reading frame of 753 base pair. The gene shares high identity to its homologues present in different Bacillus species. The expression plasmid carrying a tact aiiA-PEBA gene was constructed and the gene was overproduced in Escherichia coli BL21 (DE3). The product expressed resulted in attenuation and suspension of the infection of Pectobacterium carotovorum subsp. carotovorum on carrot. This study verified the existence of the aiiA gene in B. amyloliquefaciens and provided a prospect of the strain as biocontrol agents with quorum quenching property on bacterial disease control.
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Acknowledgments
We thank the anonymous reviewers and Dr. Shien Lu in Department of Entomology and Plant Pathology, Mississippi State University for their valuable suggestion and revising the manuscript. This work was supported by grants from the National Natural Science Foundation of China (30972367) and the Advanced Postdoctoral Science Foundation of Shandong Province (200603059).
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Xiang-Tian Yin and Liang Xu contributed equally.
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Yin, XT., Xu, L., Fan, SS. et al. Isolation and characterization of an AHL lactonase gene from Bacillus amyloliquefaciens . World J Microbiol Biotechnol 26, 1361–1367 (2010). https://doi.org/10.1007/s11274-010-0308-8
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DOI: https://doi.org/10.1007/s11274-010-0308-8