Adherence and invasion of avian pathogenic Escherichia coli to avian tracheal epithelial cells

  • Rosa María Ramírez
  • Yolanda Almanza
  • Santos García
  • Norma Heredia
Original Paper


Avian septicemia is a systemic disease where bacteria attach and invade the avian respiratory tract and enter the bloodstream and vital organs. Avian pathogenic Escherichia coli (APEC) cause this extraintestinal disease utilizing several virulence factors that have been identified. Adhesion to the trachea is the critical initial step for septic APEC pathogenicity. We investigated the ability of APEC to associate with models of tracheal epithelium. The microorganism was able to adhere to an avian tracheal explant model of infection. In addition, a primary cell culture, derived from chicken tracheal epithelium, was developed and demonstrated the ability of APEC to attach to and invade avian tracheal cells in vitro. These results are compatible with the nature of the disease and are important to the understanding of the initial point of entry of APEC in the avian model of septic infections.


Colisepticemia Avian pathogenic Escherichia coli Adherence Invasion 



This work was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT) grant 300593-B. Rosa M. Ramirez and Yolanda Almanza were CONACYT fellows. We thank Carlos Eslava from Universidad Nacional Autónoma de México for serotyping of APEC strain.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Rosa María Ramírez
    • 1
  • Yolanda Almanza
    • 1
  • Santos García
    • 2
  • Norma Heredia
    • 2
  1. 1.Departamento de Enfermedades Infecciosas, Unidad Académica de Biología ExperimentalUniversidad Autónoma de ZacatecasGuadalupeMexico
  2. 2.Departamento de Microbiología e Inmunología, Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónSan NicolasMexico

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