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Tumor-inhibitory and liver-protective effects of Phellinus igniarius extracellular polysaccharides

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Abstract

The tumor-inhibitory and liver-protective effects of crude extracellular polysaccharides (EPS) extracted from the liquid mycelial culture of the mushroom Phellinus igniarius were studied in mice. The mice were injected with murine sarcoma S180 and murine hepatoma H22. Crude EPS at 100, 200, 400 mg kg−1 body weight was administered to EPS groups each day in the twelve consecutive days. The result showed that EPS 200 mg kg−1 body weight significantly inhibited S180 and H22 at 65.0 and 46.3%, respectively. Moreover, EPS could not only keep the numbers of WBC, RBC, PLT and the concentration of HGB in a normal range, but also normalize the activities of AST, ALT and ALP. For example, in EPS-treated mice, AST significantly reduced with the percentage of A/G reverse in S180 (P < 0.05) and H22 (P < 0.01) when the mice took EPS 200 mg kg−1 body weight. In conclusion, it was remarkable that P. igniarius EPS exhibited antitumor activity related to dosage and protected liver function by sustaining the blood routine as well as keeping the blood biochemical indexes normal.

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Abbreviations

A/G:

The amount percentage of albumin and globulin

ALP:

Alkaline phosphatase

ALT:

Alanine aminotransferace

AST:

Aspartate aminotransferace

CTX:

Cyclophosphamide

EPS:

Extracellular polysaccharides

HGB:

Hemoglobins

NS:

Normal saline

PLT:

Platelets

RBC:

Red blood cells

WBC:

White blood cells

References

  • Bursch W, Schulte-Hermann R (1986) Cytoprotective effect of the prostacyclin derivative iloprost against liver cell death induced by the hepatotoxins carbon tetrachloride and bromobenzene. Klin Wochenschr 64:47–50

    CAS  Google Scholar 

  • Chang ZQ, Oh BC, Rhee MH, Kim KS, Kim JC, Lee SP, Jo WS, Park SC (2007) Polysaccharides isolated from Phellinus baumii stimulate murine splenocyte proliferation and inhibit the lipopolysaccharide-induced nitric oxide production in RAW264.7 murine macrophages. World J Microbiol Biotechnol 23:723–727. doi:10.1007/s11274-006-9277-3

    Article  CAS  Google Scholar 

  • Chang ZQ, Hwang MH, Rhee MH, Kim KS, Kim JC, Lee SP, Jo WS, Park SC (2008) The in vitro anti-platelet, antioxidant and cellular immunity activity of Phellinus gilvus fractional extracts. World J Microbiol Biotechnol 24:181–187. doi:10.1007/s11274-007-9454-z

    Article  Google Scholar 

  • Chen W, He FY, Li YQ (2006) The apoptosis effect of hispolon from Phellinus linteus (Berkeley and Curtis) Teng on human epidermoid KB cells. J Ethnopharmacol 105:280–285. doi:10.1016/j.jep.2006.01.026

    Article  CAS  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356. doi:10.1021/ac60111a017

    Article  CAS  Google Scholar 

  • Han SB, Lee CW, Jeon YJ, Hong ND, Yoo ID, Yang KH, Kim HM (1999) The inhibitory effect of polysaccharides isolated from Phellinus linteus on tumor growth and metastasis. Immunopharmacology 41:157–164. doi:10.1016/S0162-3109(98)00063-0

    Article  CAS  Google Scholar 

  • Hoose T, Toru I, Yoshihiko C, Takeshi I, Hiroshi M, Takayuki I, Kawai KI (2006) Relaxing effects of Phellinus gilvus extract and purified ebricoic acid on rat aortic rings. J Nat Med 60:130–134. doi:10.1007/s11418-006-0030-x

    Article  Google Scholar 

  • Ikekawa T, Nakanishi M, Uehara N, Chihara G, Fukuoka F (1968) Antitumor action of some Basidiomycetes, especially Phellinus linteus. Gann 59:155–157

    CAS  Google Scholar 

  • Jeon TI, Hwang SG, Lim BO, Park DK (2003) Extracts of Phellinus linteus grown on germinated brown rice suppress liver damage induced by carbon tetrachloride in rats. Biotechnol Lett 25:2093–2096. doi:10.1023/B:BILE.0000007071.28105.c1

    Article  CAS  Google Scholar 

  • Kim SY, Shon YH, Lee JS, Kim CH, Nam KS (2000) Antimutagenic activity of soybeans fermented with basidiomycetes in Ames/Salmonella test. Biotechnol Lett 22:1197–1202. doi:10.1023/A:1005697515592

    Article  CAS  Google Scholar 

  • Kim DH, Yang BK, Jeong SC, Park JB, Cho SP, Das Surajit, Yun JW, Song CH (2001) Production of a hypoglycemic, extracellular polysaccharide from the submerged culture of the mushroom, Phellinus linteus. Biotechnol Lett 23:513–517. doi:10.1023/A:1010312513878

    Article  CAS  Google Scholar 

  • Kim GY, Oh YH, Park YMA (2003) Acidic polysaccharide isolated from Phellinus linteus induces nitric oxide-mediated tumoricidal activity of macrophages through protein tyrosine kinase and protein kinase C. Biochem Biophys Res Commun 309:399–407. doi:10.1016/j.bbrc.2003.08.018

    Article  CAS  Google Scholar 

  • Kojima H, Tanigawa N, Kariya S, Komemushi A, Shomura Y, Sawada S, Arai E, Yokota Y (2006) A case of spontaneous regression of hepatocellular carcinoma with multiple lung metastases. Radiat Med 24:139–142. doi:10.1007/BF02493281

    Article  Google Scholar 

  • Ohno T, Takahashi Y, Tanabe H, Hirayama H, Mmizukami H, Ogihara Y, Inoue M (2007) Inhibitory effect of oral intake of natural Phellinus linteus fruit body on growth and pulmonary metastasis of B16/BL6 melanoma. J Nat Med 61:438–442. doi:10.1007/s11418-007-0163-6

    Article  Google Scholar 

  • Shon YH, Nam KS (2001) Antimutagenicity and induction of anticarcinogenic phase II enzymes by basidiomycetes. J Ethnopharmacol 77:103–109. doi:10.1016/S0378-8741(01)00276-8

    Article  CAS  Google Scholar 

  • Shon YH, Nam KS (2002) Cancer chemoprevention: inhibitory effect of soybeans fermented with basidiomycetes on 7,12-dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate-induced mouse skin carcinogenesis. Biotechnol Lett 24:1005–1010. doi:10.1023/A:1015673229528

    Article  CAS  Google Scholar 

  • Shon YH, Nam KS (2004) Inhibition of cytochrome P450 isozymes and ornithine decarboxylase activities by polysaccharides from soybeans fermented with Phellinus igniarius or Agrocybe cylindracea. Biotechnol Lett 26:159–163. doi:10.1023/B:BILE.0000012900.63039.10

    Article  CAS  Google Scholar 

  • Zou X, Sun M, Guo X (2006) Quantitative response of cell growth and polysaccharide biosynthesis by the medicinal mushroom Phellinus linteus to NaCl in the medium. World J Microbiol Biotechnol 22:1129–1133

    Article  CAS  Google Scholar 

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Acknowledgments

We thank the Association for Science and Technology Consultant Management of Shandong Province (ASTCMSD) for the financial support (No. 620427).

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Authors and Affiliations

  1. Shandong Province Key Lab of Agricultural Applied Mycology, Qingdao Agricultural University, Changcheng Road 700, Chengyang, 266109, Qingdao, People’s Republic of China

    Wei Dong, Li Ning, Wei-dong Lu, Cui-cui Li, Rui-peng Chen, Xiao-ning Jia, Lin Wang & Li-zhong Guo

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  1. Wei Dong
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  2. Li Ning
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  3. Wei-dong Lu
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  4. Cui-cui Li
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  7. Lin Wang
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  8. Li-zhong Guo
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Correspondence to Li-zhong Guo.

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Dong, W., Ning, L., Lu, Wd. et al. Tumor-inhibitory and liver-protective effects of Phellinus igniarius extracellular polysaccharides. World J Microbiol Biotechnol 25, 633–638 (2009). https://doi.org/10.1007/s11274-009-9967-8

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  • DOI: https://doi.org/10.1007/s11274-009-9967-8

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