Abstract
Streptomyces rimosus CN08 isolated from Tunisian soil produced 8.6 mg l−1 of oxytetracycline (OTC) under submerged fermentation (SmF). Attempts were made for enhancing OTC production after irradiation-induced mutagenesis of Streptomyces rimosus CN08 with Co60-γ rays. 125 OTC-producing colonies were obtained after screening on kanamycin containing medium. One mutant called Streptomyces rimosus γ-45 whose OTC production increased 19-fold (165 mg l−1) versus wild-type strain was selected. γ-45 mutant was used for OTC production under solid-state fermentation (SSF). Wheat bran (WB) was used as solid substrate and process parameters influencing OTC production were optimized. Solid-state fermentation increased the yield of antibiotic production (257 mg g−1) when compared with submerged fermentation. Ammonium sulphate as additional nitrogen source enhanced OTC level to 298 mg g−1. Interestingly, OTC production by γ-45 mutant was insensitive to phosphate which opens the way to high OTC production even in medium containing phosphate necessary for optimal mycelia growth.
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Acknowledgments
This work was supported by the “Ministère de l’Enseignement Supérieur de la Recherche Scientifique et de la Technologie” of Tunisia. We thank Prof. Ezzedine Aouani for valuable discussion and critical reading of the manuscript.
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Hadeer Lazim and Nedra Slama contributed equally to this work.
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Lazim, H., Slama, N., Mankai, H. et al. Enhancement of oxytetracycline production after gamma irradiation-induced mutagenesis of Streptomyces rimosus CN08 strain. World J Microbiol Biotechnol 26, 1317–1322 (2010). https://doi.org/10.1007/s11274-009-0303-0
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DOI: https://doi.org/10.1007/s11274-009-0303-0