Abstract
Accumulation of trehalose in yeasts has been suggested to be an important mechanism of tolerance against adverse stress conditions, particularly in thermal stress. However, under thermal stress, it is not clear if the mechanism of protection is related to its antioxidant role. In this study, a newly isolated wine yeast Saccharomyces cerevisia was used to examine the protective effect of trehalose against oxidation during thermal stress treatment. Cells were treated either with a mild heat treatment at 37°C (which leads to trehalose accumulation) or with a 50 mM trehalose solution and then exposed to a high temperature of 53°C. According to our results, mild heat treatment at 37°C and trehalose addition which promote accumulation of trehalose significantly increased cell survival upon exposure to thermal stress at 53°C which seems to be correlated with decrease in reactive oxygen species levels and lipid peroxidation. Trehalose could protect yeast from oxidative injuries under thermal stress.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aebi H (1984) Catalase in vitro. Meth Enzymol 105:121–126
Allison SD, Chang B, Randolph TW, Carpenter JF (1999) Hydrogen bonding between sugar and protection is responsible for inhibition of dehydration-induced protein unfolding. Arch Biochem Biophys 365:289–298
Almeselmani M, Deshmukh PS, Sairam RK, Kushwaha SR, Singh TP (2006) Protective role of antioxidant enzymes under high temperature stress. Plant Sci 171:382–388. doi:10.1016/j.plantsci.2006.04.009
Ansari A, Jones CM, Henry ER, Hofrichter J, Eaton WA (1992) The Role of solvent viscosity in the dynamics of protein conformational changes. Science 256:1796–1798
Asker MMS, Ramadan MF, El-Aal SKA, El-Kady EMM (2009) Characterization of trehalose synthase from Corynebacterium nitrilophilus NRC. World J Microbiol Biotechnol 25:789–794. doi:10.1007/s11274-008-9950-9
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Cakmak I, Marschner H (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol 98:1222–1227
Cardona F, Carrasco P, Pérea-Ortín JE, del Olmo ML, Aranda A (2007) A novel approach for the improvement of stress resistance in wine yeasts. Int J Food Microbiol 114:83–91. doi:10.1016/j.ijfoodmicro.2006.10.043
Carvalheiro F, Roseiro JC, Gírio FM (1999) Interactive effects of sodium chloride and heat shock on trehalose accumulation and glycerol production by Saccharomyces cerevisiae. Food Microbiol 16:543–550
Cavazza A, Grando MS, Zini C (1992) Rilevazione della flora microbica di mosti e vini. Vignevini (9):17–20
Chiou TJ, Chu ST, Tzeng WF (2003) Protection of cells from menadione-induced apoptosis by inhibition of lipid peroxidation. Toxicology 191:77–88. doi:10.1016/S0300-483X(03)00189-6
Costa V, Moradas-Ferreira P (2001) Oxidative stress and signal transduction in Saccharomyces cerevisiae: insights into ageing, apoptosis and diseases. Mol Aspects Med 22:217–246
Díaz MJ, Ruiz E, Romero I, Cara C, Moya M, Castro E (2009) Inhibition of Pichia stipitis fermentation of hydrolysates from olive tree cuttings. World J Microbiol Biotechnol 25:891–899. doi:10.1007/s11274-009-9966-9
Ferreira JC, Thevelein JM, Hohmann S, Paschoalin VMF, Trugo LC, Panek AD (1997) Trehalose accumulation in mutants of Saccharomyces cerevisiae deleted in UDPG-dependent trehalose synthase-phosphatase complex. Biochim Biophys Acta 1335:40–50
Fillinger S, Chaveroche MK, van Dijck P, de Vries R, Ruijter G, Thevelein J (2001) Trehalose is required for the acquisition of tolerance to a variety of stresses in the filamentous fungus Aspergillus nidulans. Microbiology 147(7):1851–1862
Halliwell B (2006) Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol 141:312–322. doi:10.1104/pp.106.077073
Herdeiro RS, Pereira MD, Panek AD, Eleutherio ECA (2006) Trehalose protects Saccharomyces cerevisiae from lipid peroxidation during oxidative stress. Biochim Biophys Acta 1760:340–346. doi:10.1016/j.bbagen.2006.01.010
Herrero E, Ros J, Bellí G, Cabiscol E (2008) Redox control and oxidative stress in yeast cells. Biochim Biophys Acta 1780:1217–1235. doi:10.1016/j.bbagen.2007.12.004
Ivannikova YV, Naumova ES, Naumov GI (2007) Viral dsRNA in the wine yeast Saccharomyces bayanus var. uvarum. Res Microbiol 158:638–643. doi:10.1016/j.resmic.2007.07.008
Jepsen HF, Jensen B (2004) Accumulation of trehalose in the thermophilic fungus Chaetomium thermophilum var. coprophilum in response to heat or salt stress. Soil Biol Biochem 36:1669–1674. doi:10.1016/j.soilbio.2004.07.010
Kandror O, Bretschnelder N, Kreydln E, Cavallerl D, Goldberg AL (2004) Yeast adapt to near-freezing temperatures by STRE/Msn2, 4-Dependent induction of trehalose synthesis and certain molecular chaperones. Mol Cell 13:771–781
Luo Y, Li WM, Wang W (2008) Trehalose: protector of antioxidant enzymes or reactive oxygen species scavenger under heat stress? Environ Exp Bot 63:378–384. doi:10.1016/j.envexphot.2007.11.016
Marullo P, Bely M, Masneuf-Pomarede I, Aigle M, Dubourdieu D (2004) Inheritable nature of enological quantitative traits is demonstrated by meiotic segregation of industrial wine yeast strains. FEMS Yeast Res 4:711–719. doi:10.1016/j.femsyr.2004.01.006
Masneuf-Pomarède I, Mansour C, Murat M-L, Tominaga T, Dubourdieu D (2006) Influence of fermentation temperature on volatile thiols concentrations in Sauvibnon blanc wines. Int J Food Microbiol 108:385–390. doi:10.1016/j.ijfoodmicro.2006.01.001
Pallmann CL, Brown JA, Olineka TL, Cocolin L, Mills DA, Bisson LF (2001) Use of WL medium to profile native flora fermentations. Am J Enol Vitic 52(3):198–203
Parrou JL, Teste MA, Francios J (1997) Effects of various types of stress on the metabolism of reserve carbohydrates in Saccharomyces cerevisiae: genetic evidence for a stress-induced recycling of glycogen and trehalose. Microbiology 143:1891–1900
Patterson BD, Macrae EA, Ferguson IB (1984) Estimation of hydrogen peroxide in plant extracts using titanium (IV). Anal Biochem 139:487–492
Plourde-Owobi L, Durner S, Goma G, Francois J (2000) Trehalose reserve in Saccharomyces cerevisiae: phenomenon of transport, accumulatin and role in cell viability. Int J Food Microbiol 55:33–40
Richards AB, Krakowka S, Dexter LB, Schmid H, Wolterbeek APM, Waalkens-Berendsen DH, Shigoyuki A, Kurimoto M (2002) Trehalose: a review of properties, history of use and human tolerance, and results of multiple safety studies. Food Chem Toxicol 40(7):871–898
Shao HB, Chu LY, ZhH Lu, Kang CM (2008) Primary antioxidant free radical scavenging and redox signaling pathways in higher plant cells. Int J Biol Sci 4(1):8–14
Singer MA, Lindquist S (1998) Thermotolerance in Saccharomyces cerevisiae: the Yin and Yang of trehalose. Trends Biotechnol 16:460–468
Wang AG, Luo GH (1990) Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiol Commun (6):55–57
Wen PF, Chen JY, Kong WF, Pan QH, Wan SB, Huang WD (2005) Salicylic acid induced the expression of phenylalanine ammonia-lyase gene in grape berry. Plant Sci 169:928–934. doi:10.1016/j.plantsci.2005
Acknowledgments
This work was supported by National Natural Science Foundation of China (No. 30611468) and major program of Beijing Municipal Science & Technology Commission (No. D07060500160701).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, H., Wang, HL., Du, J. et al. Trehalose protects wine yeast against oxidation under thermal stress. World J Microbiol Biotechnol 26, 969–976 (2010). https://doi.org/10.1007/s11274-009-0258-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-009-0258-1